...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Metallic oxide nanoparticles stimulate blood coagulation independent of their surface charge
【24h】

Metallic oxide nanoparticles stimulate blood coagulation independent of their surface charge

机译：金属氧化物纳米粒子不依赖于表面电荷而刺激血液凝结

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Positively charged metallic oxides prevent blood coagulation whereas negatively charged metallic oxides are thrombogenic. This study was performed to examine whether this effect extends to metallic oxide nanoparticles. Oscillation shear rheometry was used to study the effect of zinc oxide and silicon dioxide nanoparticles on thrombus formation in human whole blood. Our data show that oscillation shear rheometry is a sensitive and robust technique to analyze thrombogenicity induced by nanoparticles. Blood without previous contact with nanoparticles had a clotting time (CT) of 16.7 ± 1.0 min reaching a maximal clot strength (CS) of 16 ± 14 Pa (G') after 30 min. ZnO nanoparticles (diameter 70 nm, +37 mV zeta-potential) at a concentration of 1 mg/mL prolonged CT to 20.8 ± 3.6 min and provoked a weak clot (CS 1.5 ± 1.0 Pa). However, at a lower concentration of 100 μg/mL the ZnO particles dramatically reduced CT to 6.0 ± 0.5 min and increased CS to 171 ± 63 Pa. This procoagulant effect decreased at lower concentrations reaching the detection limit at 10 ng/mL. SiO2 nanoparticles (diameter 232 nm, -28 mV zeta-potential) at high concentrations (1 mg/mL) reduced CT (2.1 ± 0.2 min) and stimulated CS (249 ± 59 Pa). Similar to ZnO particles, this procoagulant effect reached a detection limit at 10 ng/mL. Nanoparticles in high concentrations reproduce the surface charge effects on blood coagulation previously observed with large particles or solid metal oxides. However, nanoparticles with different surface charges equally well stimulate coagulation at lower concentrations. This stimulation may be an effect which is not directly related to the surface charge.
机译：带正电的金属氧化物可防止血液凝结，而带负电的金属氧化物可引起血栓形成。进行这项研究以检查这种作用是否扩展到金属氧化物纳米颗粒。振荡剪切流变法用于研究氧化锌和二氧化硅纳米粒子对人全血中血栓形成的影响。我们的数据表明，振荡剪切流变法是分析纳米颗粒引起的血栓形成性的灵敏且稳定的技术。之前未与纳米颗粒接触的血液的凝结时间（CT）为16.7±1.0分钟，在30分钟后达到最大凝块强度（CS）为16±14 Pa（G'）。浓度为1 mg / mL的ZnO纳米颗粒（直径70 nm，+ 37 mVζ电位）将CT延长至20.8±3.6分钟，并引起了弱凝块（CS 1.5±1.0 Pa）。但是，在100μg/ mL的较低浓度下，ZnO颗粒可将CT显着降低至6.0±0.5分钟，而CS则增加至171±63 Pa。在较低浓度下达到10 ng / mL的检出限时，促凝作用降低。高浓度（1 mg / mL）的SiO2纳米颗粒（直径232 nm，-28 mVζ电位）降低了CT（2.1±0.2分钟）并刺激了CS（249±59 Pa）。与ZnO颗粒相似，这种促凝作用达到了10 ng / mL的检测极限。高浓度的纳米颗粒可再现以前在大颗粒或固态金属氧化物中观察到的对血液凝固的表面电荷效应。然而，具有不同表面电荷的纳米粒子在较低浓度下同样能很好地促进凝结。该刺激可以是与表面电荷不直接相关的效应。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号