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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Differential response of human osteoblast-like cells to commercially pure (cp) titanium grades 1 and 4.
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Differential response of human osteoblast-like cells to commercially pure (cp) titanium grades 1 and 4.

机译：人类成骨细胞样细胞对商业纯钛（cp）1级和4级的差异反应。

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摘要

Common dental implants are made of different grades of commercially pure titanium (cpTi) that are more than 99% similar in chemical composition. The objective of this in vitro study was to determine if human osteoblast-like cells, Saos-2, would respond differently when plated on disks of cpTi Grade 1 and Grade 4. Glass disks served as controls. In spite of identical preparation, the two grades of cpTi acquired different surface topographies, as illustrated by scanning electron micrographs and profilometry. Cell responses, such as adhesion, morphology, and collagen synthesis also differed on the two grades of cpTi. Between 4 and 24 h, the rate of cell attachment to Grade 1 differed significantly compared to cell attachment to Grade 4 and to glass. Rhodamine phalloidin fluorescence microscopy showed variations in the actin-based cytoskeleton between grades 1 and 4 cpTi in cell spreading, shape, and the organization of stress fibers. Immunofluorescent staining showed differential expression of vinculin, a focal adhesion protein, on the substrates. At 24 h, the percent of collagen synthesized was significantly more on Grade 1 than on Grade 4 and on glass. Alkaline phosphatase activity was similar on all substrates. The calcium content was significantly higher on Grade 1 than on Grade 4 and on glass at 24 h and at 4 weeks. Thus, commonly used cpTi induced differential morphologic and phenotypic changes in human osteoblast-like cells depending on the grade of the material.
机译：普通的牙科植入物由不同等级的商业纯钛（cpTi）制成，其化学成分相似度超过99％。这项体外研究的目的是确定将人类成骨细胞样细胞Saos-2接种到cpTi 1级和4级圆盘上时是否会有不同反应。玻璃圆盘用作对照。尽管制备方法相同，但两个等级的cpTi仍具有不同的表面形貌，如扫描电子显微照片和轮廓测定法所示。在两个等级的cpTi上，细胞反应（如粘附，形态和胶原合成）也有所不同。在4到24小时之间，与1级电池和4级玻璃的附着率明显不同。罗丹明鬼笔环肽荧光显微镜显示，肌动蛋白基细胞骨架在1和4 cpTi级之间在细胞扩散，形状和应力纤维的组织方面存在差异。免疫荧光染色显示基质上粘着蛋白vincolin的差异表达。在24小时时，合成的胶原蛋白的百分比在1级比在4级和玻璃上要高得多。碱性磷酸酶活性在所有底物上均相似。在24小时和4周时，1级钙的含量显着高于4级钙和玻璃的钙含量。因此，根据材料的等级，常用的cpTi会在人成骨细胞样细胞中诱导不同的形态和表型变化。

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