...
首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Cytokine expression in vitro by cultured human endothelial cells in contact with polyethylene terephthalate coated with pyrolytic carbon and collagen.
【24h】

Cytokine expression in vitro by cultured human endothelial cells in contact with polyethylene terephthalate coated with pyrolytic carbon and collagen.

机译:培养的人内皮细胞与涂有热解碳和胶原的聚对苯二甲酸乙二酯接触,在体外表达细胞因子。

获取原文
获取原文并翻译 | 示例
           

摘要

In order to evaluate whether or not polyethylene terephthalate coated with pyrolytic carbon and collagen (PET+PC) favors inflammatory or hyperplastic reactions, the expression of mRNAs specific for interleukin-6 (IL-6), platelet-derived growth factor-A (PDGF-A), PDGF-B, transforming growth factor-beta1 (TGF-beta1), and TGF-beta2 were tested in vitro by cultured human umbilical vein endothelial cells (HUVEC). The cultures were put in contact with PET+PC for 1, 24, 48, and 72 h. The same cells cultured on polystyrene without biomaterials were tested as negative controls; cultures incubated with LPS were the positive control. The expression of mRNAs was evaluated by RT-PCR with specific primers. PET+PC did not determine any differences in the expression of IL-6-specific mRNA at any of the incubation times compared to the negative control while LPS (the positive control) induced expression after 24, 48, and 72 h. PET+PC induced a more precocious expression of mRNA specific for PDGF-A than did the negative control; however, the expression no longer was present after 48 h while in the negative control the expression stopped after 72 h. PET+PC induced a less frequent expression of PDGF-B-specific mRNA than did the negative control and LPS, especially after 24 h. PET+PC induced a later expression of TGF-beta2-specific mRNA than did the negative control and a less frequent expression of mRNA specific for TGF-beta1 after 24 and 72 h. Copyright 2000 John Wiley & Sons, Inc.
机译:为了评估涂有热解碳和胶原蛋白的聚对苯二甲酸乙二醇酯(PET + PC)是否有助于炎症或增生性反应,对白细胞介素6(IL-6),血小板源性生长因子-A(PDGF)特异的mRNA表达-A),PDGF-B,转化生长因子-beta1(TGF-beta1)和TGF-beta2在体外通过培养的人脐静脉内皮细胞(HUVEC)进行了测试。使培养物与PET + PC接触1、24、48和72小时。测试了在没有生物材料的聚苯乙烯上培养的相同细胞作为阴性对照。用LPS孵育的培养物为阳性对照。通过RT-PCR用特异性引物评估mRNA的表达。与阴性对照相比,PET + PC在任何温育时间均未确定IL-6特异性mRNA表达的任何差异,而LPS(阳性对照)在24、48和72小时后诱导表达。与阴性对照相比,PET + PC诱导PDGF-A特异的mRNA表达更加早熟。然而,在48小时后该表达不再存在,而在阴性对照中72小时后该表达停止。与阴性对照和LPS相比,PET + PC诱导的PDGF-B特异性mRNA表达的频率降低,尤其是在24小时后。与阴性对照相比,PET + PC诱导TGF-β2特异性mRNA的表达较晚,而在24和72小时后,TGF-β1特异性mRNA的表达频率较低。版权所有2000 John Wiley&Sons,Inc.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号