...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Mouse fibroblasts in long-term culture within collagen three-dimensional scaffolds: influence of crosslinking with diphenylphosphorylazide on matrix reorganization, growth, and biosynthetic and proteolytic activities.
【24h】

Mouse fibroblasts in long-term culture within collagen three-dimensional scaffolds: influence of crosslinking with diphenylphosphorylazide on matrix reorganization, growth, and biosynthetic and proteolytic activities.

机译：在胶原三维支架中长期培养的小鼠成纤维细胞：与二苯基磷酰叠氮化物交联对基质重组，生长以及生物合成和蛋白水解活性的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

With the rapid development of tissue engineering and gene therapy, collagen-based biomaterials frequently are used as cell transplant devices. In this study we determined the behavior of mouse fibroblasts cultured for up to 6 weeks in control sponges treated by severe dehydration and used commercially as hemostatic agents and in two sponges (DPPA 2 and 3) crosslinked by diphenylphosphorylazide, a method developed in our laboratory. Growth capacity, biosynthetic and proteolytic activities, and matrix reorganization were followed over time in cultures and compared with similar data for fibroblasts in monolayer culture on plastic and in floating or attached collagen gels. Control sponges with and without seeded mouse fibroblasts showed rapid partial denaturation or contraction, weight loss, and severe calcification (13-18% Ca) after 6 weeks. In contrast, the crosslinked sponges showed only slightly decreased size and weight, and the calcification was inhibited (0.2% Ca) in the presence of cells. Mouse fibroblasts seeded on the crosslinked sponge surface at 50,000-200,000 cells/cm(2) progressively penetrated the matrix and proliferated to give the same constant cell density after 3 weeks (around 600,000 cells/sponge). A specific, two- to threefold decrease in collagen synthesis was observed between 1 and 3 or 6 weeks, due mainly to a decrease in the fraction secreted into the medium (25-30% instead of 45-50%). No collagenase 3 activity was detected in the culture medium under any condition or time whereas 25% gelatinase A was found by gelatin zymography to be in an active form in cultures within sponges as compared with less than 10% in monolayers and more than 50% in floating collagen gel. A small amount of gelatinase B was observed after 1 week in sponge cultures and was completely absent thereafter. These results show that the biosynthetic and proteolytic behavior of mouse fibroblasts cultured in crosslinked collagen scaffolds is different from that in monolayers or in floating collagen gels and more similar to that previously described in attached collagen gels. Copyright 2000 John Wiley & Sons, Inc.
机译：随着组织工程和基因疗法的迅速发展，基于胶原的生物材料经常被用作细胞移植装置。在本研究中，我们确定了在经过严格脱水处理的对照海绵中培养长达6周的小鼠成纤维细胞的行为，该海绵在商业上用作止血剂，并在通过本实验室开发的二苯基磷酰叠氮化物交联的两个海绵（DPPA 2和3）中进行了测定。随着时间的推移，对培养物的生长能力，生物合成和蛋白水解活性以及基质重组进行了追踪，并将其与塑料，漂浮或附着的胶原蛋白凝胶的单层培养中成纤维细胞的相似数据进行了比较。有和没有种子小鼠成纤维细胞的对照海绵在6周后显示出快速的局部变性或收缩，体重减轻和严重的钙化（13-18％Ca）。相反，交联的海绵仅显示出略微减小的尺寸和重量，并且在存在细胞的情况下抑制了钙化（0.2％Ca）。以50,000-200,000个细胞/ cm（2）的密度接种在交联的海绵表面上的小鼠成纤维细胞逐渐穿透基质，并在3周后增殖以提供相同的恒定细胞密度（约60万个细胞/海绵）。在1至3或6周内观察到胶原蛋白合成的特异性降低了2到3倍，这主要是由于分泌到培养基中的级分减少了（25-30％，而不是45-50％）。在任何条件或时间下，在培养基中均未检测到胶原酶3活性，而通过明胶酶谱法发现海绵中的培养物中有25％的明胶酶A以活性形式存在，而在单层中不到10％，在单层中超过50％。漂浮的胶原蛋白凝胶。 1周后在海绵培养物中观察到少量明胶酶B，此后完全不存在。这些结果表明，在交联的胶原蛋白支架中培养的小鼠成纤维细胞的生物合成和蛋白水解行为与单层或漂浮胶原蛋白凝胶中的不同，并且与先前在附着的胶原蛋白凝胶中描述的相似。版权所有2000 John Wiley＆Sons，Inc.

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号