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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Ultra-high molecular weight polyethylene oxidation reduces metalloproteinase 2 secretion in human osteoblast-like cells in vitro: A mechanism of modulation of extracellular matrix
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Ultra-high molecular weight polyethylene oxidation reduces metalloproteinase 2 secretion in human osteoblast-like cells in vitro: A mechanism of modulation of extracellular matrix

机译：超高分子量聚乙烯氧化可在体外减少人成骨样细胞中金属蛋白酶2的分泌：一种调节细胞外基质的机制

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Ultra-high molecular weight polyethylene (UHMWPE) sterilization with gamma rays induced high oxidation levels both on the surface and in the bulk that alter its structure and mechanical properties. The oxidation process of gamma-radiated UHMWPE induces a reduction of molecular weight and, consequently, a less abrasive resistance that has been related, among others, to the failure of UHMWPE in vivo. To explain the role of cells in such events, human osteoblast-like cells were seeded onto UHMWPE and oxidized UHMWPE discs. Cellular viability and morphology were evaluated along with matrix metalloproteinases (MMPs) production and activity. Oxidized UHMWPE did not induce any significant cytotoxic effects as observed by lactate dehydrogenase activity compared to the nonoxidized form; no changes in the cell morphology after 4 and 8 days proliferation were observed. In growth medium metalloproteinase 2 (gelatinase-A, MMP-2) was produced and released by osteoblast-like cells. We observed that cells grown onto oxidized UHMWPE discs decreased the release and activity of MMP-2 after 4 and 8 days culture compared to cells grown on control and non-oxidized UHMWPE discs; metalloproteinase 9 (gelatinase-B, MMP-9) release was not significantly influenced. The absence of cytotoxic and morphological effects in the presence of a down-regulation of MMP-2 release and activity suggest that oxidized polyethylene surfaces may modulate matrix remodeling and, consequently, bone formation.
机译：用伽马射线进行的超高分子量聚乙烯（UHMWPE）灭菌会在表面和整体中引起高氧化水平，从而改变其结构和机械性能。 γ射线辐照的UHMWPE的氧化过程导致分子量降低，因此，耐磨性降低，这尤其与体内UHMWPE的失败有关。为了解释细胞在此类事件中的作用，将人类成骨细胞样细胞接种到UHMWPE盘上并氧化UHMWPE盘。评估细胞活力和形态以及基质金属蛋白酶（MMP）的产生和活性。与未氧化的形式相比，氧化的UHMWPE不会通过乳酸脱氢酶活性观察到任何明显的细胞毒性作用。增殖4天和8天后未观察到细胞形态的变化。在生长培养基中产生金属蛋白酶2（明胶酶-A，MMP-2）并由成骨细胞样细胞释放。我们观察到，与在对照和非氧化UHMWPE光盘上生长的细胞相比，在氧化的UHMWPE光盘上生长的细胞在培养4天和8天后降低了MMP-2的释放和活性。金属蛋白酶9（明胶酶-B，MMP-9）的释放未受到明显影响。在MMP-2释放和活性下调的情况下，没有细胞毒性和形态学作用表明，氧化的聚乙烯表面可能会调节基质重塑，进而导致骨形成。

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