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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Expression of nitric oxide, peroxynitrite, and apoptosis in loose total hip replacements
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Expression of nitric oxide, peroxynitrite, and apoptosis in loose total hip replacements

机译：一氧化氮，过氧亚硝酸盐和细胞凋亡在全髋关节置换中的表达

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Nitric oxide (NO) is an effector molecule associated with inflammation, immune function, bone metabolism, and the induction of apoptosis. This study examined the role of NO, peroxynitrite (ONOO~-), and apoptosis in cases of revision total hip replacements (THRs). We hypothesized that apoptosis and excess production of NO contribute to the inflammatory reaction to orthopedic biomaterial wear debris that is associated with loosening and osteolysis. Periprosthetic membranous specimens were collected from revised cemented acetabular components with simple loosening and ballooning osteolysis. Synovial samples from patients undergoing primary THR were used as controls. The presence of macrophages (CD68~+) and levels of inducible nitric oxide synthase (INOS), endothelial nitric oxide synthase (EcNOS), ONOO~- (Nitro, assayed by the amount of nitrated tyrosine residues), and apoptosis (TUNEL staining) were examined using immunohistochemistry. Increased expression for INOS, EcNOS, and ONOO~- in both the loose/osteolytic and the looseon-osteolytic groups was observed when compared to the synovium group. There were no significant differences between the loose/osteolytic group and looseon-osteolytic group for these biologic markers. TUNEL staining showed a significant increase in apoptosis in the loose/osteolytic group compared to the looseon-osteolytic group and synovial tissues. These findings suggest that NO and NO-derived molecules, such as ONOO~-, may be involved in sustaining the foreign-body reaction to wear debris. NO and ONOO~- may prove to be useful markers of prosthetic loosening whereas apoptosis may be a marker distinguishing ballooning from simple osteolysis.
机译：一氧化氮（NO）是与炎症，免疫功能，骨骼代谢和细胞凋亡诱导相关的效应分子。这项研究检查了NO，过氧亚硝酸盐（ONOO〜-）和细胞凋亡在翻修全髋关节置换术（THRs）中的作用。我们假设细胞凋亡和NO的过量产生导致与骨科生物材料磨损碎片相关的炎症反应，这些碎片与松弛和骨溶解有关。从改良的骨水泥髋臼组件中收集骨膜周围膜标本，并进行简单的松动和膨胀性溶骨。来自接受原发性THR的患者的滑膜样品用作对照。巨噬细胞（CD68〜+）的存在和诱导型一氧化氮合酶（INOS），内皮型一氧化氮合酶（EcNOS），ONOO〜-（硝基，通过硝化酪氨酸残基的含量测定）和细胞凋亡（TUNEL染色）的水平使用免疫组织化学检查。与滑膜组相比，在松散/溶骨组和松散/非溶骨组中均观察到INOS，EcNOS和ONOO-的表达增加。在这些生物标志物的松/溶骨组和松/非溶骨组之间没有显着差异。 TUNEL染色显示，与疏松/非溶骨组和滑膜组织相比，疏松/溶骨组的细胞凋亡显着增加。这些发现表明，NO和NO衍生的分子（如ONOO-）可能参与维持异物对磨损碎片的反应。 NO和ONOO-可能被证明是假体松动的有用标志物，而细胞凋亡可能是区别于单纯溶骨性气球样变的标志物。

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