...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Inhibition of CEM calcification by the sequential pretreatment with ethanol and EDTA
【24h】

Inhibition of CEM calcification by the sequential pretreatment with ethanol and EDTA

机译：乙醇和EDTA顺序预处理对CEM钙化的抑制作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The major object of the present study is to optimize the anticalcification activity of ethanol on bioprosthetic heart valve (BHV) calcification. We hypothesize that the chelating agent, in combination with ethanol, will synergistically prevent aortic wall calcification. Collagen-elastin matrix (CEM) was developed as a calcifiable matrix for simulating the calcification process of implantable biomaterials. The efficacy of the combination effects of ethanol and EDTA on the calcification process of GEMs was investigated by implanting them after pretreatment with various conditions of ethanol and EDTA in the rat subdermal model. The relationship between calcium concentrations and pretreatment conditions (a series vs. simultaneous, i.e., first ethanol and then EDTA in water solution, the reverse, or EDT in ethanol) was established and the optimal condition for prevention of BHV calcification was determined. The mechanistic studies on anticalcification effects exerted by particular pretreatment sequences were also conducted using FTIR and differential scanning calorimetry (DSC). The sequential pretreatment of CEM first with ethanol and then EDTA in water solution significantly decreased the calcification rate of CEM compared the control. The percentage of prevention of calcification by the serial treatment of ethanol (80 percent v/v) and then EDTA in water solutions decreased, as the concentration of elastin in the CEM increased. The percentage of preventing calcification was 42 percent, 28.6 percent, and 22.9 percent for CEM containing collagen and elastin ratios of 90:10, 50:50, 20:80, respectively. These results indicate that elastin is the major regulatory component of BHV calcification, and preventive effects on calcification increased only when CEM were pretreated with first ethanol and then EDTA in water solution. Moreover, the sequential effect is more apparent in the matrix of less elastin content, which is close to the physiological range. The sequential inhibitory effects of ethanol and EDTA could occur due to the distinct separate actions of each agent, thereby achieving a relatively greater inhibition of calcification.
机译：本研究的主要目的是优化乙醇对生物人工心脏瓣膜（BHV）钙化的抗钙化活性。我们假设螯合剂与乙醇结合将协同预防主动脉壁钙化。胶原蛋白弹性蛋白基质（CEM）被开发为可模仿的基质，用于模拟可植入生物材料的钙化过程。乙醇和EDTA联合作用对GEMs钙化过程的功效是通过在大鼠皮下模型中用各种条件的乙醇和EDTA预处理后将它们植入而研究的。建立了钙浓度与预处理条件之间的关系（一系列对比同时显示，即先加入乙醇然后再加入EDTA的水溶液，相反的溶液或乙醇中的EDT），并确定了预防BHV钙化的最佳条件。还使用FTIR和差示扫描量热法（DSC）对特定预处理序列产生的抗钙化作用进行了机理研究。与对照相比，先用乙醇然后用EDTA对CEM进行顺序预处理可以显着降低CEM的钙化率。随着CEM中弹性蛋白浓度的增加，先后用乙醇（80％v / v）和EDTA依次处理来防止钙化的百分比降低。对于含有90：10、50：50、20：80的胶原蛋白和弹性蛋白比例的CEM，预防钙化的百分比分别为42％，28.6％和22.9％。这些结果表明，弹性蛋白是BHV钙化的主要调节成分，只有在先将CEM先用乙醇然后在水溶液中进行EDTA预处理时，对钙化的预防作用才会增强。而且，在弹性蛋白含量较低的基质中，顺序效应在接近生理范围的基质中更为明显。乙醇和EDTA的顺序抑制作用可能是由于每种试剂的不同单独作用而发生的，从而实现了相对较大的钙化抑制作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号