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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >In vitro apatite induction by osteopontin: Interfacial energy for hydroxyapatite nucleation on osteopontin
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In vitro apatite induction by osteopontin: Interfacial energy for hydroxyapatite nucleation on osteopontin

机译：骨桥蛋白诱导体外磷灰石：界面能在骨桥蛋白上羟化磷灰石成核

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摘要

Noncollagenous phosphoproteins that interact with type I collagen are thought to nucleate the mineral phase to the collagen fibril network of mineralized tissues. We previously reported that a specific dentin phosphoprotein, phosphophoryn, crosslinked to an insoluble substrate such as type I collagen fibrils, was an effective nucleator of apatite. In this study, we investigated the capacity of another phosphoprotein in dentin, osteopontin, for apatite nucleation in vitro. Osteopontin purified from bovine milk was either absorbed to agarose beads or crosslinked to agarose beads. Each preparation was incubated at 37 deg C in metastable solutions. Apatite was induced by osteopontin that was crosslinked to agarose beads, whereas osteopontin adsorbed to agarose beads failed to induce apatite crystal formation. Using classical nucleation theory, the interfacial energy for hydroxyapatite nucleation on osteopontin crosslinked to agarose beads was determined to be 94.7 ergs/cm~2. This value is larger than that for phosphophoryn, though it is similar to that for hydroxyapatite. The present study indicates that osteopontin, like phosphophoryn, has a high potential to induce apatite formation when it is covalently bound to some substrate in vitro, and suggests the possibility that osteopontin bound to type I collagen may induce apatite formation in vivo.
机译：与I型胶原蛋白相互作用的非胶原磷蛋白被认为可将矿物质相成核到矿化组织的胶原纤维网络中。我们先前曾报道过，一种特定的牙本质磷蛋白磷光蛋白可与不溶性基质（如I型胶原纤维）交联，是磷灰石的有效成核剂。在这项研究中，我们调查了牙本质中另一种磷酸化蛋白骨桥蛋白在体外磷灰石成核的能力。从牛乳中纯化的骨桥蛋白被吸收到琼脂糖珠上或交联到琼脂糖珠上。每种制剂在亚稳溶液中于37摄氏度孵育。骨桥蛋白交联到琼脂糖珠上诱导磷灰石，而吸附在琼脂糖珠上的骨桥蛋白则不能诱导磷灰石晶体形成。利用经典的成核理论，确定了与琼脂糖珠交联的骨桥蛋白上羟基磷灰石成核的界面能为94.7 ergs / cm〜2。尽管该值类似于羟基磷灰石的值，但该值大于磷磷蛋白的值。本研究表明，骨桥蛋白与磷光蛋白一样，在体外与某些底物共价结合时，具有很高的诱导磷灰石形成的潜力，并暗示与我I型胶原结合的骨桥蛋白可能在体内诱导磷灰石形成。

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