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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Utilizing Acid Pretreatment and Electrospinning to Improve Biocompatibility of Poly(Glycolic Acid) for Tissue Engineering
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Utilizing Acid Pretreatment and Electrospinning to Improve Biocompatibility of Poly(Glycolic Acid) for Tissue Engineering

机译：利用酸预处理和静电纺丝技术改善组织工程中聚乙醇酸的生物相容性

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Poly(glycolic acid) (PGA) has a long history as a bioresorbable polymer. Its biocompatibility is widely accepted, yet PGA is often rejected as a soft-tissue scaffold because of fibrous encapsulation. The goal of this study was to improve the soft-tissue biocompatibility of PGA by producing scaffolds composed of small-diameter fibers through electrospinning and subjecting these scaffolds to a concentrated hydrochloric acid (HCL) pretreatment. The theory is that small-diameter fibers will elicit a reduced immune response and HC1 treatment will improve cellular interactions. Scaffolds were characterized in terms of fiber diameter and pore area via image-analysis software. Biocompatibility was assessed through a WST-1 cell-proliferation assay (in vitro) with the use of rat cardiac fibroblasts and rat intramuscular implantations (in vivo). Fibers produced ranged in diameter from 0.22 to 0.88 pun with pore areas from 1.84 to 13.22 mu m~2. The untreated scaffold composed of 0.88-mu m~2 fibers was encapsulated in vivo and supported the lowest rates of cell proliferation. On the contrary, the acid pretreated scaffold with 0.22-mu m fibers was incorporated into the surrounding tissue and exhibited proliferation rates that exceeded the control populations on tissue-culture plastic. In conclusion, this study has shown the ability to improve the biocompatibility of PGA through acid pretreatment of scaffolds comprised of submicron fiber diameters.
机译：聚乙醇酸（PGA）作为可生物吸收的聚合物历史悠久。它的生物相容性已被广泛接受，但由于纤维包裹，PGA通常不被视为软组织支架。这项研究的目的是通过静电纺丝生产由小直径纤维组成的支架，并对这些支架进行浓盐酸（HCL）预处理，从而提高PGA的软组织生物相容性。从理论上讲，小直径的纤维将引起免疫应答降低，而HCl处理将改善细胞相互作用。通过图像分析软件，根据纤维直径和孔面积表征支架。通过使用大鼠心脏成纤维细胞和大鼠肌肉内植入（体内），通过WST-1细胞增殖试验（体外）评估生物相容性。所生产的纤维的直径范围为0.22至0.88pun，孔面积为1.84至13.22μm〜2。将未经处理的由0.88μm〜2纤维组成的支架包裹在体内，并支持最低的细胞增殖速率。相反，将经过酸预处理的具有0.22μm纤维的支架掺入周围的组织中，其增殖速率超过了组织培养塑料上的对照种群。总之，这项研究表明，通过对包含亚微米纤维直径的支架进行酸预处理，可以提高PGA的生物相容性。

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