...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Buffer solution can control the porosity of DNA-chitosan complexes.
【24h】

Buffer solution can control the porosity of DNA-chitosan complexes.

机译：缓冲溶液可以控制DNA-壳聚糖复合物的孔隙率。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The current studies examine the pore properties and biological effects of DNA-chitosan complexes, which may be useful as scaffolds for tissue engineering. The porosity of the DNA-chitosan complexes was controlled by rinsing them with several different pH 7.2 buffer solutions, including phosphate-buffered saline (PBS), Tris-HCl, boric acid, and N-(2-hydroxyethyl)piperazine-N'-(2-ethanesufonic acid) (HEPES). Rinsing with PBS resulted in 84% porosity, whereas rinsing with Tris-HCl produced 94% porosity. It was further found that daunorubicin hydrochloride complex intercalated with and bound to the groove of the DNA-chitosan complexes, indicating that DNA in the complexes maintains its double-stranded helical structure. The DNA-chitosan complexes were not toxic to MG-63 osteoblast-like cells and caused only a mild tissue response when implanted subcutaneously in the backs of rats. These results suggest that buffer-rinsed DNA-chitosan complexes may be useful as a scaffold material in tissue engineering.
机译：当前的研究检查了脱氧核糖核酸-壳聚糖复合物的孔隙特性和生物学效应，这可作为组织工程的支架。通过使用几种不同的pH 7.2缓冲溶液（包括磷酸盐缓冲液（PBS），Tris-HCl，硼酸和N-（2-羟乙基）哌嗪-N'- （2-乙磺酸）（HEPES）。用PBS冲洗产生的孔隙度为84％，而使用Tris-HCl冲洗产生的孔隙度为94％。进一步发现盐酸柔红霉素复合物嵌入并结合到DNA-壳聚糖复合物的凹槽中，表明复合物中的DNA保持其双链螺旋结构。 DNA-壳聚糖复合物对MG-63成骨细胞样细胞无毒，皮下植入大鼠背部仅引起轻度的组织反应。这些结果表明，缓冲液冲洗的DNA-壳聚糖复合物可用作组织工程中的支架材料。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号