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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Metal ion-induced toxic histamine release from human basophils and mast cells.
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Metal ion-induced toxic histamine release from human basophils and mast cells.

机译：金属离子诱导的毒性组胺从人类嗜碱细胞和肥大细胞释放。

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Recent data suggest that distinct metal ions can be released from dental alloys or other biomaterials, and may cause toxic effects on various cells. In this study, the effects of 14 metal ions on histamine release from human blood basophils (n = 4), isolated tissue mast cells (lung n = 8, uterus n = 2, skin n = 1, gingiva n = 1), the basophil cell line KU-812, and the mast cell line HMC-1 were analyzed. Of the 14 metal ions, Ag+ (0.33 mM) and Hg2+ (0.33 mM) were found to induce release of histamine in blood basophils, KU-812, mast cells, and HMC-1. The effects of Ag+ and Hg2+ were dose dependent and were observed within 60 min of incubation. In primary mast cells and basophils, AU3+ (0.33 mM) also induced histamine release, whereas no effects of Au3+ on HMC-1 or KU-812 cells were seen. The other metal ions showed no effects on primary or immortal cells within 60 min. However, Pt4+ (0.33 mM) induced histamine liberation in HMC-1 and lung mast cells after 12 h. The Ag+- and Hg2+-induced rapid release of histamine from HMC-1 was associated with ultrastructural signs of necrosis, but not apoptosis. In contrast, prolonged exposure to Pt4+ (0.33 mM, 14 h) induced apoptotic cell death in HMC-1 cells, as assessed by electron microscopy and DNA analysis. Together, certain metal ions induce distinct cytopathogenic effects in mast cells and basophils. Whereas Ag+, Hg2+, and Au3+ cause direct toxicity, Pt4 causes cell death through induction of apoptosis. Whether such effects contribute to local adverse reactions to metal-containing biomaterials in vivo remains to be determined.
机译：最新数据表明，可以从牙科合金或其他生物材料中释放出独特的金属离子，并且可能对各种细胞产生毒性作用。在这项研究中，14种金属离子对人血嗜碱性粒细胞（n = 4），分离的组织肥大细胞（肺n = 8，子宫n = 2，皮肤n = 1，牙龈n = 1），组织胺释放的组胺的影响分析嗜碱性粒细胞系KU-812和肥大细胞系HMC-1。在14种金属离子中，发现Ag +（0.33 mM）和Hg2 +（0.33 mM）诱导嗜碱性粒细胞，KU-812，肥大细胞和HMC-1中组胺的释放。 Ag +和Hg2 +的作用是剂量依赖性的，在孵育60分钟内即可观察到。在原代肥大细胞和嗜碱性粒细胞中，AU3 +（0.33 mM）也诱导组胺释放，而未观察到Au3 +对HMC-1或KU-812细胞的影响。其他金属离子在60分钟内对原代或永生细胞无影响。然而，Pt4 +（0.33 mM）诱导HMC-1和肺肥大细胞在12小时后释放组胺。 Ag +和Hg2 +诱导的HMC-1组胺快速释放与坏死的超微结构体征有关，但与细胞凋亡无关。相反，通过电子显微镜和DNA分析评估，长时间暴露于Pt4 +（0.33 mM，14 h）会诱导HMC-1细胞凋亡。某些金属离子共同在肥大细胞和嗜碱性粒细胞中诱导不同的细胞致病作用。 Ag +，Hg2 +和Au3 +引起直接毒性，而Pt4通过诱导凋亡导致细胞死亡。这种作用是否会导致体内对含金属生物材料的局部不良反应尚待确定。

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