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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Flow cytometric analysis of macrophage response to ceramic and polyethylene particles: effects of size, concentration, and composition.
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Flow cytometric analysis of macrophage response to ceramic and polyethylene particles: effects of size, concentration, and composition.

机译：巨噬细胞对陶瓷和聚乙烯颗粒反应的流式细胞仪分析：大小，浓度和组成的影响。

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Using the J774 macrophage cell line, we designed an in vitro model to analyze by flow cytometry the effects of size, concentration, and composition of ceramic (Al2O3 and ZrO2) and high density polyethylene (HDP) particles on phagocytosis and cell mortality. Inflammatory mediator (TNF-alpha) also was measured by ELISA. Kinetic studies revealed that phagocytosis of the particles begins very early after cell exposure, increasing with time and particle concentration and reaching a plateau after 15 h. This implies that the optimum period to evaluate cellular response to particulate debris is between 15 and 24 h of incubation. Results also showed that phagocytosis increases with concentration for particles up to 2 microns. For larger particles (up to 4.5 microns), phagocytosis seems to reach a plateau independent of size and concentration, which suggests a saturation of phagocytosis that is most likely dependent on overall particle volume ingested. We did not detect any significant difference in phagocytosis between Al2O3 and ZrO2 at 0.6 microns. Al2O3 seems to be more easily phagocytosed than HDP at the same size (4.5 microns) and concentrations. Cytotoxicity studies revealed that macrophage mortality increases with particle size and concentration for sizes greater than 2 microns. Smaller particles (0.6 microns) cause cell mortality only at higher concentrations (from 1,250 particles per cell), but the mortality is still very low (10%). No significant difference in cell mortality and TNF-alpha release was found between Al2O3 and ZrO2. Effects of Al2O3 and HDP at 4.5 microns were compared by measuring TNF-alpha release. Results showed that TNF-alpha release increases with particle concentrations and is higher with HDP than with Al2O3.
机译：我们使用J774巨噬细胞系设计了体外模型，通过流式细胞术分析了陶瓷（Al2O3和ZrO2）和高密度聚乙烯（HDP）颗粒的尺寸，浓度和组成对吞噬作用和细胞死亡的影响。炎症介质（TNF-α）也通过ELISA测量。动力学研究表明，颗粒的吞噬作用在暴露于细胞后很早就开始，随时间和颗粒浓度的增加而增加，并在15小时后达到平稳。这意味着评估细胞对颗粒碎片的反应的最佳时间为孵育15至24小时。结果还表明，吞噬作用随浓度增加而增加，最高达2微米。对于较大的颗粒（最大4.5微米），吞噬作用似乎达到了一个与大小和浓度无关的平稳状态，这表明吞噬作用的饱和度很可能取决于所摄入的总颗粒体积。我们没有发现在0.6微米处Al2O3和ZrO2之间的吞噬作用有任何显着差异。在相同尺寸（4.5微米）和相同浓度下，Al2O3似乎比HDP更容易被吞噬。细胞毒性研究表明，对于粒径大于2微米的颗粒，巨噬细胞死亡率随粒径和浓度的增加而增加。较小的颗粒（0.6微米）仅在较高浓度时会导致细胞死亡（每个细胞1,250个颗粒），但死亡率仍然非常低（10％）。 Al2O3和ZrO2之间的细胞死亡率和TNF-α释放没有显着差异。通过测量TNF-α释放，比较了Al2O3和HDP在4.5微米处的影响。结果表明，TNF-α释放随颗粒浓度增加而增加，HDP比Al2O3高。

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