High density of immobilized galactose ligand enhances hepatocyte attachment and function

Chao Yin; Lei Ying; Peng-Chi Zhang

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High density of immobilized galactose ligand enhances hepatocyte attachment and function

机译：高密度的固定半乳糖配体可增强肝细胞的附着和功能

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Galactosylated surface is an attractive substrate for hepatocyte culture because of the specific interaction between the galactose ligand and the asialoglycoprotein receptor on hepatocytes. In this study, we described a scheme to achieve high density of immobilized galactose ligands on polyethylene terephthalate (PET) surface by first surface-grafting polyacrylic acid on plasma-pretreated PET film under UV irradiation, followed by conjugation of a galactose derivative (1-O-(6'-aminohexyl)-D-galactopyranoside) to the grafted polyacrylic acid chains. A high galactose density of 513 nmol/cm~2 on the PET surface was used in this study to investigate the behavior of cultured hepatocyte. This engineered substrate showed high affinity to fluorescein isothiocyanate-lectin binding. Primary rat hepatocytes, when seeded at a density of 2 X 10~5 cells/cm~2, attached to the galactosylated PET substrate at a similar efficiency compared with collagen-coated substrate. The hepatocytes spontaneously formed aggregates 1 day after cell seeding and showed better maintenance of albumin secretion and urea synthesis functions than those cultured on collagen-coated surface.

机译：半乳糖基化的表面是肝细胞培养的诱人底物，因为半乳糖配体与肝细胞上的去唾液酸糖蛋白受体之间存在特异性相互作用。在这项研究中，我们描述了一种通过首先在紫外线照射下将聚丙烯酸表面等离子处理的PET膜表面接枝，然后缀合半乳糖衍生物（1-）来在聚对苯二甲酸乙二醇酯（PET）表面上实现高密度的固定半乳糖配体的方案。 O-（6'-氨基己基）-D-吡喃半乳糖苷）到接枝的聚丙烯酸链上。本研究以PET表面半乳糖的高密度513 nmol / cm〜2来研究培养的肝细胞的行为。该工程化的底物对荧光素异硫氰酸酯-凝集素结合表现出高亲和力。以2 X 10〜5细胞/ cm〜2的密度接种时，原代大鼠肝细胞以与胶原蛋白包被的底物相似的效率附着于半乳糖基化的PET底物。接种细胞后1天，肝细胞自发形成聚集体，与在胶原蛋白涂层表面上培养的相比，它们显示出更好的维持白蛋白分泌和尿素合成功能。

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《Journal of biomedical materials research. Part B, Applied biomaterials.》 |2003年第4期|共12页
作者
Chao Yin; Lei Ying; Peng-Chi Zhang;
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正文语种 eng
中图分类医用一般科学;
关键词
galactosylated surface; immobilization; ligand; hepatocyte; function maintenance;

机译：半乳糖基化表面;固定化;配体;肝细胞;功能维持;

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专利

1. High density of immobilized galactose ligand enhances hepatocyte attachment and function [J] . Chao Yin, Lei Ying, Peng-Chi Zhang Journal of biomedical materials research. Part B, Applied biomaterials. . 2003,第4期

机译：高密度的固定半乳糖配体可增强肝细胞的附着和功能
2. Immobilization of tripeptide growth factor glycyl-L-histidyl-L-lysine on poly(vinylalcohol)-quarternized stilbazole (PVA-SbQ) and its use as a ligand for hepatocyte attachment. [J] . Kawase M, Kurikawa N, Masuda K, Biological & pharmaceutical bulletin . 1999,第9期

机译：三肽生长因子甘氨酰-L-组氨酸-L-赖氨酸在聚（乙烯醇）-季铵化的噻唑（PVA-SbQ）上的固定及其作为肝细胞附着的配体的用途。
3. Immobilized E-cadherin model can enhance cell attachment and differentiation of primary hepatocytes but not proliferation [J] . Nagaoka M, Ise H, Akaike T Biotechnology Letters . 2002,第22期

机译：固定化的E-钙粘着蛋白模型可以增强原代肝细胞的细胞附着和分化，但不能增强增殖
4. BIOFUNCTIONAL SURFACE WITH HIGH DENSITY GALACTOSE LIGAND ENHANCES HEPATOCYTE ATTACHMENT AND FUNCTION [C] . Chao Yin, Lei Ying, Ren-Xi Zhuo, Proceedings of the 29th Annual Meeting of the Controlled Release Society . 2002

机译：具有高密度半乳糖配体的双功能表面增强了肝细胞的附着和功能
5. Density functional theory studies of ligand attachment to undecagold clusters. [D] . Spivey, Kasi. 2007

机译：密度泛函理论研究配体与未分解簇的结合。
6. Enhancement of galactose/N-acetylgalactosamine receptor activity on the surface of freshly isolated rat hepatocytes: evidence for masking of receptor sites by inhibitors derived from collagenase preparations. [O] . N L Stults, Y C Lee 1986

机译：新鲜分离的大鼠肝细胞表面半乳糖/ N-乙酰半乳糖胺受体活性的增强：胶原酶制剂抑制剂掩盖受体位点的证据。
7. Remarkable Enhancement in Extraction of Trivalent fBlock Elements Using a Macrocyclic Ligand with Four Diglycolamide Arms: Synthesis, Extraction, and Spectroscopic and Density Functional Theory Studies [O] . -1

机译：使用宏环配体提取三价Fblock元件的显着增强，其具有四甲酰胺臂：合成，提取和光谱和密度泛函理论研究

High density of immobilized galactose ligand enhances hepatocyte attachment and function

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