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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >A New Model of Implant-Related Osteomyelitis in Rats
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A New Model of Implant-Related Osteomyelitis in Rats

机译：大鼠植入相关性骨髓炎的新模型

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摘要

Infection related to osteosynthesis often has dramatic consequences for the patient. Prolonged hospitalization with systemic antibiotic therapy, several revision procedures, possible amputation, and even death may occur. To investigate the pathology of infection in orthopedic surgery, a new rat model of implant related osteomyelitis was developed. Three different concentrations (10~6, 10~3, and 10~2 colony-forming units (CFU)/10 mu l) of Staphylococcus aureus were inoculated into the tibial medullary cavity with simultaneous insertion of a titanium Kirschner wire. Controls received phosphate-buffered saline (PBS). Each group consisted of 10 animals. Animals were followed for 4 weeks until sacrifice. X-rays of the tibiae were taken weekly, blood counts were analyzed, and body temperature and weight were determined. After sacrifice, infection was evaluated by histological and microbiological investigations. All animals inoculated with Staph. aureus in either concentration developed microbiological, histological, and radiological signs of osteomyelitis in correlation to the amount of inoculated bacteria. X-rays clearly revealed osseous destruction after 14 days with progression of osteomyelitis during the following weeks. CFU/g bone and bone weight after sacrifice showed dependence on the amount of inoculated CFU. The histological results confirmed the radiological findings. No significant changes in blood counts, body weight, and body temperature between the groups could be observed. The results demonstrate that it is possible to develop a model of implant-related osteomyelitis in rats with dependence on the amount of inoculated bacteria. No other promoters of infection besides intramedullary insertion of titanium Kirschner wires were used in this model.
机译：与骨合成相关的感染通常会对患者产生严重后果。全身抗生素治疗延长了住院时间，进行了多次翻修，可能会截肢甚至死亡。为了研究整形外科感染的病理学，开发了一种新的大鼠植入物相关性骨髓炎模型。将三种不同浓度（10〜6、10〜3和10〜2个菌落形成单位（CFU）/ 10μl）的金黄色葡萄球菌接种到胫骨髓腔中，同时插入钛克氏针。对照组接受磷酸盐缓冲盐水（PBS）。每组由10只动物组成。跟踪动物4周直至处死。每周对胫骨进行X射线检查，对血球计数进行分析，并确定体温和体重。处死后，通过组织学和微生物学研究评估感染。所有动物均接种葡萄球菌。两种浓度的金黄色葡萄球菌均会引起骨髓炎的微生物学，组织学和放射学症状，这与接种细菌的数量有关。 X射线清楚地显示，在接下来的几周内，随着骨髓炎的进展，骨破坏已达14天。处死后的CFU / g骨骼和骨骼重量显示出对接种的CFU量的依赖性。组织学结果证实了放射学发现。两组之间的血球计数，体重和体温没有明显变化。结果表明，有可能建立依赖于接种细菌数量的大鼠植入物相关性骨髓炎模型。在该模型中，除了髓内插入钛克氏针外，没有其他感染促进剂。

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