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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Immunocytochemical identification of Type I collagen in acid-etched dentin
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Immunocytochemical identification of Type I collagen in acid-etched dentin

机译：酸蚀牙本质中I型胶原的免疫细胞化学鉴定

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摘要

The hypothesis tested in this study is that the application of phosphoric acid prevents collagen fibrils (CF) from maintaining their structural morphology, as assessed by their immunochemical antigenicity. Dentin was conditioned with EDTA and with 35 percent H_3PO_4 for 15, 30, and 60 s. For a control there was no treatment. A pre-embedding immunohistochemical procedure was evaluated under high-resolution SEM and a postembedding immunolocalization technique was performed on ultrathin sections at TEM level. Conditioning with EDTA resulted in a weaker labeling signal than for dentin conditioned with H_3PO_4. The most intense labeling was observed after dentin had been etched with H_3PO_4 for 15 s. The least intensive labeling was evident when the acid was applied for 30 s or for 60 s. A very weak signal was detected on the untreated dentin surface. These results provide evidence that a 15-s application of 35 percent H_3PO_4 causes mineral dissolution of the crystals enveloping the superficial CF without damaging the ultrastructure of the CF while longer applications of 35 percent H_3PO_4 cause alterations in the CF that decrease their antigenicity.
机译：在这项研究中测试的假设是，磷酸的应用可防止胶原纤维（CF）保持其结构形态，这是通过其免疫化学抗原性评估的。牙本质用EDTA和35％的H_3PO_4调节15、30和60 s。对于对照没有治疗。在高分辨率SEM下评估了包埋前的免疫组织化学程序，并在TEM水平对超薄切片进行了包埋后的免疫定位技术。与用H_3PO_4修饰的牙本质相比，用EDTA修饰的结果导致标记信号更弱。用H_3PO_4腐蚀牙本质15 s后，观察到最强烈的标记。当施加酸30 s或60 s时，标记强度最低。在未经处理的牙本质表面检测到非常弱的信号。这些结果提供了证据，表明15％的H_3PO_4的施加会导致包裹浅层CF的晶体发生矿物溶解而不会破坏CF的超微结构，而35％的H_3PO_4的较长时间的施加会导致CF的改变，从而降低其抗原性。

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