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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Plasma-polymerized surfaces for culture of human keratinocytes and transfer of cells to an in vitro wound-bed model
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Plasma-polymerized surfaces for culture of human keratinocytes and transfer of cells to an in vitro wound-bed model

机译：血浆聚合表面用于培养人角质形成细胞并将细胞转移至体外创面模型

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The aim of this study was to develop plasma-polymerized surfaces suitable for the attachment and culture of human keratinocytes and that would allow their subsequent transfer to a wound-bed model. Keratinocyte attachment has been assessed on a carrier polymer, either untreated or treated with a hydrocarbon plasma polymer, collagen I, or carboxylic-acid-containing plasma copolymers. Cell attachment was poor on the "bare" carrier polymer and hydrocarbon plasma polymer (PP) surfaces. Cell attachment was good and comparable on collagen I-coated carrier polymer and carrier polymer plasma coated with carboxylic acid functionalities. After 24 h of cell culture, surfaces were inverted so that cells were adjacent to a deepidermalized dermis (DED) for 4 days. After 4 days in contact with DED, the surfaces were removed and the level of residual cells and cells transferred to DED were assessed using a cell viability assay. Cell transfer from the collagen I-coated surface was on the order of 90 percent. Transfer from the carrier polymer surface and the hydrocarbon-coated surface was poor while cells cultured on acid-containing surfaces showed high levels of transfer. Cell transfer was greatest from those surfaces containing the highest level of acid functionality (ca. 21 percent). Cell transfer was not significantly affected by the choice of carrier polymer material although some sample-to-sample variation was seen. To determine that plasma-polymerized surfaces could be used clinically, selected samples were sterilized with ethylene oxide. Subsequent analysis and cell culture indicated that the surface chemistry and cell-transfer capability of these plasma-polymerized surfaces were unaffected by the sterilization procedure. Plasma-polymerized carboxylic-acid-containing surfaces show great promise in the field of wound healing, encouraging keratinocyte attachment and permitting keratinocyte transfer to a wound bed.
机译：这项研究的目的是开发适用于人类角质形成细胞附着和培养的等离子体聚合表面，并将其随后转移至创面模型中。已经在未经处理或用烃血浆聚合物，胶原蛋白I或含羧酸的血浆共聚物处理的载体聚合物上评估了角质形成细胞的附着。在“裸露”的载体聚合物和烃等离子体聚合物（PP）表面上的细胞附着力很差。细胞附着性良好，在胶原蛋白I涂覆的载体聚合物和等离子涂覆有羧酸官能团的载体聚合物上具有可比性。细胞培养24小时后，将表面倒置，使细胞与深真皮层真皮（DED）相邻4天。与DED接触4天后，除去表面，并使用细胞活力测定法评估残留细胞的水平和转移至DED的细胞。从胶原蛋白I涂层表面的细胞转移约为90％。从载体聚合物表面和涂有碳氢化合物的表面的转移很差，而在含酸表面上培养的细胞则显示出高水平的转移。从那些含有最高水平的酸官能团（约21％）的表面，细胞转移最大。尽管发现样品之间存在一些差异，但载体聚合物材料的选择并未显着影响细胞转移。为了确定等离子体聚合的表面可用于临床，将选定的样品用环氧乙烷灭菌。随后的分析和细胞培养表明，这些等离子体聚合表面的表面化学性质和细胞转移能力不受灭菌程序的影响。血浆聚合的含羧酸表面在伤口愈合领域具有广阔的前景，可促进角质形成细胞的附着并允许角质形成细胞转移至伤口床。

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