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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Remineralization of demineralized albumin- calcium phosphate coatings
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Remineralization of demineralized albumin- calcium phosphate coatings

机译：脱盐的白蛋白-磷酸钙涂层的再矿化

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摘要

Calcium phosphate and bovine serum albumin were coprecipitated (under physiological conditions of temperature and pH) upon the surfaces of titanium-alloy samples, which thereby became coated with a dense, proteinaceous mineral layer 30-50 mum in thickness. Dissolution of the inorganic phase by treatment with acidic saline yielded a self-supporting protein scaffold, 7-10 mum in thickness. Energy-dispersive X-ray analysis and Fourier-transform infrared spectroscopy confirmed the absence of inorganic components from the demineralized albumin scaffolds. When titanium-alloy samples bearing these demineralized protein scaffolds were immersed in a supersaturated solution of calcium phosphate (again at physiological temperature and pH), they remineralized. These redux albumin-calcium phosphate layers corresponded in thickness to the original coatings. When titanium-alloy discs bearing the demineralized protein scaffolds were implanted ectopically (subcutaneously) in mice, they, too, remineralized. No uniform mineral layer was deposited upon the surfaces of naked titanium-alloy implants. To the best of our knowledge, this is the first demonstration of remineralization within the interstices of a noncollagenous protein scaffold, either in vitro or in vivo.
机译：磷酸钙和牛血清白蛋白（在温度和pH的生理条件下）共沉淀在钛合金样品的表面上，从而被厚度为30至50微米的致密的蛋白质矿物质层覆盖。通过用酸性盐水处理来溶解无机相产生了厚度为7-10μm的自支撑蛋白支架。能量色散X射线分析和傅立叶变换红外光谱法证实，脱矿白蛋白支架中不存在无机成分。当将带有这些脱矿质蛋白质支架的钛合金样品浸入过饱和的磷酸钙溶液中（同样在生理温度和pH下）时，它们会重新矿化。这些氧化白蛋白-磷酸钙层的厚度对应于原始涂层。当将带有脱矿质蛋白质支架的钛合金椎间盘异位（皮下）植入小鼠体内时，它们也会再矿化。在裸钛合金植入物的表面上没有沉积均匀的矿物层。据我们所知，这是在体外或体内非胶原蛋白支架的空隙中再矿化的第一个证明。

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