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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >In vivo biocompatibility evaluation of cellulose macrocapsules for islet immunoisolation: Implications of low molecular weight cut-off
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In vivo biocompatibility evaluation of cellulose macrocapsules for islet immunoisolation: Implications of low molecular weight cut-off

机译：胰岛免疫分离的纤维素大胶囊的体内生物相容性评估：低分子量截止的含义。

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Earlier we showed the in vitro suitability of cellulose molecular dialysis membrane of low cut-off for islet immunoisolation. Using a syngenic islet transplantation model, here we report the in vivo biocompatibility status and discuss implications of the low molecular weight cut-off of this membrane. Streptozotocin diabetic mice were transplanted ip with islet grafts in cellulose macrocapsules and monitored for 6 weeks for their blood glucose profiles. Membrane biocompatibility was evaluated by assessing islet graft morphometry, viability, functionality, and tissue reaction against the capsules. Animals (n=10) attained normoglycemia after 1 week of transplantation and remained nondiabetic throughout a follow-up of 6 weeks. Animals subjected to a glucose tolerance test at the end of the study showed delayed glycemic control, indicating a delayed insulin response. Grafts retrieved after the 15th day and after 6 weeks showed viable islets, between 70-80 percent and approx 40 percent, respectively, with morphometric parameters similar to freshly isolated islets. Graft retrievals resulted in a recurrence of hyperglycemia, indicating a functional tissue mass. A mild tissue reaction with a few immunocytes and a very thin fibrous capsular reaction were seen against the macrocapsule membrane. This study points out the in vivo suitability of cellulose membrane for islet immunoislation. However, applicability of this membrane is limited due to its low cut-off value. Membranes with a higher molecular weight cut-off may render better glycemic control and higher graft viability.
机译：先前我们显示了低截止值的纤维素分子透析膜在体外对胰岛免疫分离的适应性。使用同基因胰岛移植模型，在这里我们报告体内生物相容性状态，并讨论该膜低分子量截留的含义。将链脲佐菌素糖尿病小鼠与胰岛移植物一起腹膜内ip移植到纤维素大胶囊中，并监测其血糖状况，持续6周。通过评估胰岛移植物的形态，活力，功能性和针对胶囊的组织反应来评估膜的生物相容性。移植1周后，动物（n = 10）达到了正常血糖水平，并且在整个6周的随访中均保持非糖尿病状态。在研究结束时进行葡萄糖耐量测试的动物显示出延迟的血糖控制，表明延迟的胰岛素反应。在第15天和6周后取回的移植物显示出可行的胰岛，分别介于70％至80％和大约40％之间，其形态计量学参数与新鲜分离的胰岛相似。移植物取回导致高血糖症复发，表明功能性组织块。观察到针对大胶囊膜的轻度组织反应和少量免疫细胞以及非常薄的纤维荚膜反应。这项研究指出了纤维素膜在胰岛免疫隔离中的体内适应性。但是，该膜的截止值低，因此其适用性受到限制。截留分子量较高的膜可提供更好的血糖控制和更高的移植物活力。

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