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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >In vitro release kinetics of proteins from bioactive foams
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In vitro release kinetics of proteins from bioactive foams

机译：生物活性泡沫中蛋白质的体外释放动力学

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摘要

This study describes an approach to obtaining 3-D scaffolds for tissue engineering that allows the incorporation and release of biologically active proteins to stimulate cell function. Laminin was adsorbed on the textured surfaces of binary 70S30C (70 mol percent SiO_2, 30 mol percent CaO) and ternary 58S (60 mol percent SiO_2 36 mol percent CaO, 4 mol percent P_2O_5) foams. The covalent bonds between the binding sites of the proteins and the ligands on the scaffolds' surfaces did not denaturate the proteins. In vitro studies show that the foams modified with chemical groups and coated with laminin were bioactive, as demonstrated by the formation of a crystalline hydroxy carbonate apatite (HCA) layer formed on the surfaces of the foams upon exposure to simulated body fluid (SBF). The release of proteins from the foams also was investigated. Sustained and controlled release from the scaffolds over a 30-day period was achieved. Laminin release from the bioactive foams followed the dissolution rate of the material network. These results suggest that bioactive foams have the potential to act as scaffolds for soft-tissue engineering with a controlled release of proteins that can induce tissue formation or regeneration.
机译：这项研究描述了一种获得用于组织工程的3-D支架的方法，该支架允许掺入和释放生物活性蛋白以刺激细胞功能。层粘连蛋白被吸附在二元70S30C（70 mol％SiO_2，30 mol％CaO）和三元58S（60 mol％SiO_2 36 mol％CaO，4 mol％P_2O_5）泡沫的织构表面上。蛋白质的结合位点与支架表面上的配体之间的共价键不会使蛋白质变性。体外研究表明，经化学基团修饰并涂有层粘连蛋白的泡沫具有生物活性，这是通过在暴露于模拟体液（SBF）后在泡沫表面形成结晶的碳酸羟基磷灰石（HCA）层来证明的。还研究了蛋白质从泡沫中的释放。在30天的时间内实现了从支架的持续控制释放。从生物活性泡沫中释放层粘连蛋白遵循材料网络的溶解速率。这些结果表明，生物活性泡沫具有作为软组织工程支架的潜力，具有受控释放的蛋白质，可以诱导组织形成或再生。

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