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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Type I collagen production by osteoblast-like cells cultured in contact with different bioactive glasses
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Type I collagen production by osteoblast-like cells cultured in contact with different bioactive glasses

机译：通过与不同生物活性眼镜接触培养的成骨样细胞产生I型胶原

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Bioactive glasses are silica-based, surface-active bone substitutes that have shown good biocompatibility both in bone and in soft tissue and are used in oral and maxillofacial bone augmentation. Previous in vitro studies showing that bioactive glasses support the growth and maturation of rat osteoblast-like cells and promote the expression and maintenance of the osteoblastic phenotype have suggested that there is both a solution-mediated and a surface-controlled effect on cell activity. In this study, we investigated the behavior of human primary osteoblast-like cells cultured in contact with three different bioactive glasses and compared them with amorphous silica (SiO_2) used in the form of granules. The specific activity of alkaline phosphatase determined biochemically was significantly higher at 2 and 4 days on the bioactive glass with 46.1 mol percent silica content (45S5 Bioglass~R) cultures than in the control cultures and in the bioactive gel-glass cultures, which had 60 mol percent (58S) and 80 mol percent (77S) silica content. Osteoblasts synthesize collagen type I, which is subsequently mineralized. Immunoblot and biochemical studies showed increased collagen release from osteoblast-like cells cultured in contact with bioactive glasses over that of controls. Among the three bloactive glasses, 45S5 is the highest inducer of osteoblast-like cell collagen release; moreover, mRNA for type I collagen was stimulated approximately three- to fivefold after 45S5 treatment. 77S bioactive glass similarly increased type I collagen synthesis even though alkaline phosphatase was not higher. These results suggest that 45S5 Bioglass~R not only induces osteogenic differentiation of human primary osteoblast-like cells, but can also increase collagen synthesis and release. The newly formulated bioactive gel-glass 77S seems to have potential applications for tissue engineering, inducing increased collagen synthesis.
机译：生物活性玻璃是基于二氧化硅的表面活性骨替代物，在骨骼和软组织中均显示出良好的生物相容性，可用于口腔和颌面骨的增强。先前的体外研究表明，生物活性玻璃支持大鼠成骨细胞样细胞的生长和成熟，并促进成骨细胞表型的表达和维持，这表明对细胞活性既有溶液介导的作用，又有表面控制的作用。在这项研究中，我们调查了与三种不同生物活性玻璃接触培养的人类原代成骨细胞样细胞的行为，并将它们与以颗粒形式使用的无定形二氧化硅（SiO_2）进行了比较。在具有46.1 mol％二氧化硅含量的生物活性玻璃（45S5 Bioglass〜R）培养物中，在2天和4天时，通过生物化学测定的碱性磷酸酶的比活性显着高于对照培养物和60倍于生物活性凝胶-玻璃培养物中的碱性磷酸酶的比活性。摩尔百分比（58S）和80摩尔百分比（77S）的二氧化硅含量。成骨细胞合成I型胶原，随后被矿化。免疫印迹和生化研究表明，与对照相比，与生物活性玻璃接触培养的成骨细胞样细胞释放的胶原蛋白增加。在这三款眼镜中，45S5是成骨细胞样细胞胶原释放的最高诱导剂。此外，在45S5处理后，I型胶原的mRNA大约被刺激了三到五倍。即使碱性磷酸酶含量不高，77S生物活性玻璃也能类似地增加I型胶原蛋白的合成。这些结果表明45S5 Bioglass〜R不仅诱导人原代成骨细胞样细胞的成骨分化，而且还可以增加胶原蛋白的合成和释放。新配制的生物活性凝胶玻璃77S似乎在组织工程中具有潜在的应用，可诱导增加的胶原蛋白合成。

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