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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Modulation of differentiation and mineralization of marrow stromal cells cultured on biomimetic hydrogels modified with Arg-Gly-Asp containing peptides
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Modulation of differentiation and mineralization of marrow stromal cells cultured on biomimetic hydrogels modified with Arg-Gly-Asp containing peptides

机译：在含Arg-Gly-Asp肽修饰的仿生水凝胶上培养的骨髓基质细胞分化和矿化的调节

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摘要

We synthesized biomimetic hydrogels modified with an osteopontin-derived peptide (ODP) and used them as a substrate for in vitro culture of marrow stromal cells (MSCs) to investigate the effect of the biomimetic surface on differentiation of MSCs into osteoblasts. Proliferation and biological assays for 16 days proved that MSCs became differentiated into osteoblasts secreting osteogenic phenotypic markers such as alkaline phosphatase (ALP), osteopontin, and mineralized calcium. In addition, there was an additive effect of the cell-binding peptide on differentiation and mineralization of MSCs cultured in the presence of soluble osteogenic supplements in cell culture media. For example, calcium content at day 16 on peptide-modified hydrogels was significantly higher than on tissue culture polystyrene. Two general trends were observed: (1) proliferation of MSCs decreased as the amount of differentiation markers increased, and (2) higher peptide concentrations accelerated the differentiation of MSCs. On the hydrogel modified with ODP, ALP activity exhibited a maximum value of 36.7 +- 4.2 pmol/cell/h at day 10 for the concentration of 2 pmol/g while the culture time needed for maximum ALP activity occurred on day 13 for the lower concentrations. On the same hydrogel, the calcium content at day 10 was 21.4 +- 2.3 ng/cell for the peptide concentration of 2 mu mol/g and 1.0 +- 0.3 ng/cell for 1.0 mu mol/g. We used Gly-Arg-Gly-Asp-Ser (GRGDS) for modification of the hydrogel as a comparison to the results with ODP. However, osteoblast development was not significantly affected by the nature of the binding peptide sequences. These results suggest that MSC function can be modulated by variation of the peptide concentration in biomimetic hydrogels used for scaffold-based bone tissue engineering.
机译：我们合成了由骨桥蛋白衍生肽（ODP）修饰的仿生水凝胶，并将其用作体外培养骨髓基质细胞（MSC）的底物，以研究仿生表面对MSC分化为成骨细胞的影响。经过16天的增殖和生物学分析证明，MSC已分化为成骨细胞，分泌成骨表型标记物，如碱性磷酸酶（ALP），骨桥蛋白和矿化钙。另外，细胞结合肽对在细胞培养基中存在可溶性成骨补充剂存在下培养的MSC的分化和矿化具有附加作用。例如，肽修饰的水凝胶在第16天的钙含量显着高于组织培养的聚苯乙烯。观察到两个总体趋势：（1）随着分化标志物数量的增加，MSC的增殖减少;（2）更高的肽浓度加速了MSC的分化。在用ODP修饰的水凝胶上，浓度为2 pmol / g时，第10天的ALP活性最大值为36.7±-4.2 pmol / cell / h，而较低的ALP活性在第13天出现最大值浓度。在同一水凝胶上，第10天的钙浓度为2μmol/ g的肽浓度为21.4±2.3ng /细胞，而1.0μmol/ g的浓度为1.0±0.3ng /细胞。我们将Gly-Arg-Gly-Asp-Ser（GRGDS）用于水凝胶的修饰，以与ODP的结果进行比较。但是，成骨细胞的发育不受结合肽序列性质的影响。这些结果表明，可通过改变用于基于支架的骨组织工程的仿生水凝胶中肽浓度的变化来调节MSC功能。

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