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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Ultrasound preexposure improves endothelial cell binding and retention on biomaterial surfaces.
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Ultrasound preexposure improves endothelial cell binding and retention on biomaterial surfaces.

机译:超声预暴露可改善内皮细胞在生物材料表面的结合和保留。

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摘要

In spite of the extensive studies regarding the effects of ultrasound on biological systems, the influence of low-intensity ultrasound on endothelial cells has rarely been investigated. In this work, the effect of ultrasound in improving the binding between endothelial cells and biomaterial substrates was evaluated. Based on the results, low-intensity ultrasound could change the morphology and matrix secretion of endothelial cells, and such effects persisted when preexposed cells were seeded to another substrate. The cells preexposed to ultrasound were spread further on the substrate. The actin stress fibers of ultrasound-preexposed cells on RGD-modified surfaces were especially intense and well oriented. Ultrasound could probably activate cellular integrins and subsequently allow RGD to bind them. A much firmer adhesion of ultrasound-preexposed endothelial cells to the biomaterial surface coated with the RGD-containing protein was demonstrated. Finally, polyurethane small-diameter vascular grafts seeded with ultrasound-preexposed endothelial cells showed enhanced cell retention on graft surfaces upon flushing.
机译:尽管对超声对生物系统的影响进行了广泛的研究,但很少研究低强度超声对内皮细胞的影响。在这项工作中,评估了超声波在改善内皮细胞与生物材料基质之间的结合方面的作用。根据这些结果,低强度超声可以改变内皮细胞的形态和基质分泌,当将预先暴露的细胞接种到另一种基质上时,这种效应仍然存在。预先暴露于超声的细胞进一步散布在基板上。在RGD修饰的表面上,超声暴露的细胞的肌动蛋白应力纤维特别牢固且取向良好。超声波可能会激活细胞整合素,随后允许RGD结合它们。超声暴露的内皮细胞对覆盖有含RGD的蛋白质的生物材料表面的粘附力强得多。最后,植入有超声暴露的内皮细胞的聚氨酯小直径血管移植物在冲洗后表现出增强的细胞保留在移植物表面的能力。

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