...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Osteoblast response to titanium surface roughness and 1#alpha#,25-(OH)_2D_3 is mediated through the mitogen-activated protein kinase (MAPK) pathway
【24h】

Osteoblast response to titanium surface roughness and 1#alpha#,25-(OH)_2D_3 is mediated through the mitogen-activated protein kinase (MAPK) pathway

机译：成骨细胞对钛表面粗糙度和1＃alpha＃，25-（OH）_2D_3的反应是通过有丝分裂原激活的蛋白激酶（MAPK）途径介导的

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

When osteoblasts are cultured on surfaces of in-creasing microroughness, they exhibit decreases in proliferation, increases in differentiation and local factor production, and enhanced response to 1#alpha#,25(OH)_2D_3. The cells interact with surfaces through integrins, which signal by the same pathways used by 1#alpha#,25(OH)_2D_3, including protein kinase C via phospholipase C and protein kinase A via phospholipase A_2. This provides opportunities for crosstalk that may con-tribute to the synergistic effects of surface roughness and the vitamin D metabolite. Because these pathways converge at mitogen-activated protein kinase (MAPK), we tested the hypothesis that the extracellular signal-regulated kinase (ERK1/2) subclass of MAPKs mediates the effects of surface roughness and 1#alpha#,25(OH)_2D_3. MG63 osteoblast-like osteosarcoma cells were cultured on commercially pure Ti disks with various surface roughnesses: pretreatment (PT; 0.6 #mu#m average roughness (Ra)), coarse grit-blasted and acid-etched (SLA; 4 #mu#m RA), and titanium plasma-sprayed (TPS; 5.2-#mu#m R_a). At confluence, cells were treated for 24 h with control media or media containing 10~(-7) M 1#alpha#,25(OH)_2D_3. One-half of the cultures received 1 #mu#M or 10 #mu#M PD98059, a specific inhibitor of the ERK family of MAPKs. PD98059 alone did not affect proliferation, osteocalcin production, or production of transforming growth factor-#beta#1or nitric oxide, regardless of the surface roughness. Alkaline phosphatase was reduced by the inhibition of the ERK family kinases on all surfaces to a comparable extent. However, when PD98059 was added to the cultures with 1#alpha#,25(OH)_2D_3, the effects of the seco-steroid were blocked, including the synergistic increases seen in MG63 cells cultured on SLA or TPS. These results indicate that ERK1/2 MAPK is required for the maintenance of alkaline phosphatase at control levels and that the effects of 1#alpha#,25(OH)_2D_3 are mediated by ERK1/2. However, the effects of surface roughness are not due to the ERK family of MAPKs. This suggests that alternative pathways may be used, including those mediated by other MAPK subclasses.
机译：当成骨细胞在增加的微粗糙度表面上培养时，它们表现出增殖减少，分化和局部因子产生增加以及对1＃alpha＃，25（OH）_2D_3的响应增强。细胞通过整联蛋白与表面相互作用，整联蛋白通过与1＃α＃，25（OH）_2D_3所使用的相同途径发出信号，包括通过磷脂酶C的蛋白激酶C和通过磷脂酶A_2的蛋白激酶A。这为串扰提供了机会，可能有助于表面粗糙度和维生素D代谢产物的协同作用。因为这些途径汇聚在促分裂原活化蛋白激酶（MAPK）上，所以我们测试了MAPKs的细胞外信号调节激酶（ERK1 / 2）亚类介导表面粗糙度和1＃alpha＃，25（OH）_2D_3的影响的假设。 。将MG63成骨样骨肉瘤细胞培养在具有各种表面粗糙度的市售纯钛圆盘上：预处理（PT; 0.6＃mu＃m平均粗糙度（Ra））;粗喷砂处理和酸蚀处理（SLA; 4＃mu＃m） RA）和等离子喷涂钛（TPS;5.2-μμmR_a）。汇合时，将细胞用对照培养基或含有10〜（-7）M 1＃alpha＃，25（OH）_2D_3的培养基处理24小时。一半的培养物接受1＃mu＃M或10＃mu＃M PD98059，一种ERK MAPKs家族的特异性抑制剂。不管表面粗糙度如何，单独使用PD98059都不会影响增殖，骨钙素的产生或转化生长因子-β＃1或一氧化氮的产生。碱性磷酸酶通过在所有表面上抑制ERK家族激酶而降低到相当的程度。但是，将PD98059添加到含有1＃alpha＃，25（OH）_2D_3的培养物中时，封闭类固醇的作用被阻止，包括在SLA或TPS上培养的MG63细胞中看到的协同增加。这些结果表明ERK1 / 2 MAPK是维持碱性磷酸酶在控制水平所需的，并且1＃alpha＃，25（OH）_2D_3的作用是由ERK1 / 2介导的。但是，表面粗糙度的影响并非归因于MAPK的ERK家族。这表明可以使用其他途径，包括其他MAPK子类介导的途径。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号