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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Fibroblast growth on surface-modified dental implants: An in vitro study
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Fibroblast growth on surface-modified dental implants: An in vitro study

机译：表面修饰的牙种植体上成纤维细胞的生长：一项体外研究

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A major consideration in designing dental implants is the creation of a surface that provides strong attachment between the implant and bone, connective tissue, or epithelium. In addition, it is important to inhibit the adherence of oral bacteria on titanium surfaces exposed to the oral cavity to maintain plaque-free implants. Previous in vitro studies have shown that titanium implant surfaces coated with titanium nitride (TiN) reduced bacterial colonization compared to other clinically used implant surfaces. The aim of the present study was to examine the support of fibroblast growth by a TiN surface that has antimicrobial characteristics. Mouse fibroblasts were cultured on smooth titanium discs that were either magnetron-sputtered with a thin layer of titanium nitride, thermal oxidized, or modified with laser radiation (using a Nd-YAG laser). The resulting surface topography was examined by scanning electron microscopy (SEM), and surface roughness was estimated using a two-dimensional contact stylus profilometer. A protein assay (BCA assay) and a colorimetric assay to examine fibroblast metabolism (MTT) were used. Cellular morphology and cell spreading were analyzed using SEM and fluorescence microscopy. Fibroblasts on oxidized titanium surfaces showed a more spherical shape, whereas cells on laser-treated titanium and on TiN appeared intimately adherent to the surface. The MTT activity and total protein were significantly increased in fibroblasts cultured on titanium surfaces coated with TiN compared to all other surface modifications tested. This study suggests that a titanium nitride coating might be suitable to support tissue growth on implant surfaces.
机译：设计牙科植入物时的主要考虑因素是创建一个表面，该表面在植入物与骨骼，结缔组织或上皮之间提供牢固的附着。另外，重要的是抑制口腔细菌粘附在暴露于口腔的钛表面上，以保持无斑块的植入物。先前的体外研究表明，与其他临床使用的植入物表面相比，涂有氮化钛（TiN）的钛植入物表面减少了细菌定植。本研究的目的是检查具有抗菌特性的TiN表面对成纤维细胞生长的支持。将小鼠成纤维细胞培养在光滑的钛圆盘上，该圆盘上磁控溅射有氮化钛薄层，经过热氧化，或经激光辐照（使用Nd-YAG激光）改性。通过扫描电子显微镜（SEM）检查所得的表面形貌，并使用二维接触测针轮廓仪测量表面粗糙度。使用蛋白质测定法（BCA测定法）和比色测定法检查成纤维细胞代谢（MTT）。使用SEM和荧光显微镜分析细胞形态和细胞扩散。氧化钛表面的成纤维细胞呈球形，而经过激光处理的钛和TiN上的细胞则紧密粘附在表面。与测试的所有其他表面修饰相比，在涂有TiN的钛表面培养的成纤维细胞中，MTT活性和总蛋白显着增加。这项研究表明，氮化钛涂层可能适合支持植入物表面上的组织生长。

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