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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Cell seeding into calcium phosphate cement
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Cell seeding into calcium phosphate cement

机译：细胞接种到磷酸钙水泥中

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To improve the effectiveness of calcium phosphate cement (CPC), we have developed a method to seed osteoblasts into the cement. CPC powder is mixed with water to form a paste that can be shaped to fit a bone defect in situ. The paste hardens in 30 mm, reacts to form hydroxyapatite, and is replaced with new bone. Reacted CPC is biocompatible but unreacted CPC paste was found to have toxic effects when placed on cell monolayers (MC3T3-E1 cells). In contrast, when cells were indirectly exposed to CPC paste using a porous membrane or by placing a coverslip containing adherent cells onto a bed of CPC paste, the unreacted CPC was nontoxic. These results suggested that gel encapsulation of the cells might protect them from the CPC paste. Thus, cells were encapsulated in alginate beads (3.6-mm diameter), mixed with CPC paste, and incubated overnight. Both vital staining (calcein-AM and ethidium homodimer-1) and the Wst-1 assay (measures dehydrogenase activity) showed that cell survival in alginate beads that were mixed with CPC was similar to survival in untreated control beads. These results suggest that gel encapsulation could be used as a mechanism to protect cells for seeding into CPC.
机译：为了提高磷酸钙水泥（CPC）的有效性，我们开发了一种将成骨细胞播种到水泥中的方法。将CPC粉末与水混合形成糊状物，糊状物可以成形为适合就位的骨缺损。糊状物在30毫米内变硬，反应形成羟基磷灰石，并被新骨替代。反应后的CPC具有生物相容性，但未反应的CPC糊剂放置在细胞单层（MC3T3-E1细胞）上时，会产生毒性作用。相反，当使用多孔膜或通过将包含粘附细胞的盖玻片间接置于CPC糊料床上时，将细胞间接暴露于CPC糊料中时，未反应的CPC无毒。这些结果表明，细胞的凝胶封装可以保护它们免受CPC糊剂的侵害。因此，将细胞封装在藻酸盐珠（直径3.6毫米）中，与CPC糊状物混合，并孵育过夜。活力染色（钙黄绿素-AM和乙锭均二聚体-1）和Wst-1分析（测量脱氢酶活性）均显示，与CPC混合的藻酸盐珠粒中的细胞存活率与未经处理的对照珠粒中的存活率相似。这些结果表明凝胶封装可以用作保护细胞播种到CPC中的机制。

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