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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Proliferation and Differentiation of Osteoblasts on Biocement D Modified with Collagen Type I and Citric Acid
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Proliferation and Differentiation of Osteoblasts on Biocement D Modified with Collagen Type I and Citric Acid

机译：I型胶原和柠檬酸修饰的生物水泥D上成骨细胞的增殖和分化

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摘要

In this study, the proliferation and differentiation of rat calvarial osteoblasts cultured on either (1) calcium-phosphate bone cement Biocement D, (2) Biocement D with 2.5 percent (w/w) mineralized collagen type I, or (3) Biocement D with 2.5 percent (w/w) mineralized collagen type I and 3 percent (w/w) citric acid were investigated. Incubation of the composites in cell-culture medium resulted in a fast decrease of pH and calcium concentration as well as in an increase of phosphate concentration. Although these effects occurred with all investigated materials, the lowest extent could be observed for the citric-acid-containing composites. As shown by scanning-electron microscopy, osteoblasts adhered to the composite surfaces. Proliferation and differentiation of the cells grown on the composites were found to be reduced compared to cells grown on tissue-culture polystyrene. Cells cultured in the vicinity of the composites but without direct contact also exhibited a reduced rate of proliferation, reduced alkaline phosphatase activity, and reduced mineralization. Simulating the changes in calcium and phosphate concentration occasioned by the composites through exposing cells to EGTA and phosphate gives rise to the same effects of reducing proliferation, ALP activity, and mineralization. No indication for apoptosis in cells exposed to low calcium and high phosphate concentrations was found. The number of necrotic cells, however, increased after incubation with EGTA and phosphate. For assessment of cell-composite interactions and the success of the composites in vivo, as well as for more effective material development, it seems to be important to know how changes in microenvironmental pH and ion composition of the material affect cellular proliferation and differentiation.
机译：在这项研究中，在（1）磷酸钙骨水泥生物水泥D，（2）具有2.5％（w / w）矿化I型胶原的生物水泥D或（3）生物水泥D上培养的大鼠颅骨成骨细胞的增殖和分化。研究了含有2.5％（w / w）矿化I型胶原蛋白和3％（w / w）柠檬酸的柠檬酸。复合材料在细胞培养基中的孵育导致pH和钙浓度的快速降低以及磷酸盐浓度的增加。尽管这些影响发生在所有研究的材料上，但对于含柠檬酸的复合材料，观察到的程度最低。如扫描电子显微镜所示，成骨细胞粘附在复合材料表面上。与在组织培养聚苯乙烯上生长的细胞相比，发现在复合材料上生长的细胞的增殖和分化降低。在复合材料附近培养但未直接接触的细胞也表现出降低的增殖速率，降低的碱性磷酸酶活性和降低的矿化作用。通过将细胞暴露于EGTA和磷酸盐中来模拟复合材料引起的钙和磷酸盐浓度变化，会产生减少增殖，ALP活性和矿化的相同效果。没有发现暴露于低钙和高磷酸盐浓度的细胞凋亡的迹象。然而，与EGTA和磷酸盐一起孵育后，坏死细胞的数量增加了。为了评估细胞复合物之间的相互作用和复合物在体内的成功以及更有效的材料开发，了解微环境pH和材料离子组成的变化如何影响细胞增殖和分化似乎很重要。

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