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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >In vitro cellular response to polypyrrole-coated woven polyester fabrics: potential benefits of electrical conductivity.
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In vitro cellular response to polypyrrole-coated woven polyester fabrics: potential benefits of electrical conductivity.

机译：体外对聚吡咯涂层聚酯聚酯细胞的反应：导电性的潜在好处。

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Electrically conducting polypyrrole-treated films have recently been shown to influence the morphology and function of mammalian cells in vitro. This type of polymer represents a possible alternative biomaterial for use in vascular implantation. The present study compared the in vitro biocompatibility of the five different polyester woven fabrics having increasing levels of electrical conductivity ranging from 4.5 x 10(4) to 123 omega/square with that of low density polyethylene and polydimethylsiloxane primary reference materials. Biocompatibility was measured in terms of four different types of in vitro cellular response, including (a) an indirect and (b) a direct control organotypic culture assay using endothelial cells, (c) a polymorphonuclear (PMN) cell activation study using flow-cytometric measurements of CD11/CD18 integrin molecule expression, and (d) a semiquantification of interleukin (IL)-6 mRNA expression on monocytes/macrophages using reverse-transcriptase polymerase chain reaction. The organotypic culture study revealed that the fabrics with high levels of conductivity exhibited lower cell migration, proliferation, and viability. The PMN activation study of blood from 10 healthy adult donors demonstrated that the two most conductive fabrics were able to identify the more reactive donors. The levels of IL-6 mRNA expression by monocytes/macrophages decreased as the conductivity level of the fabrics increased. The results of the present study therefore indicate that high levels of conductivity (< 200 omega/square) on polyester fabrics are detrimental to the growth, migration, and viability of endothelial cells; induce elevated PMN activation; and affect the intracellular metabolism of monocytes. They also point to a specific range of conductivity (10(3) < 10(4) omega/square) which is associated with an optimum in vitro cellular response.
机译：导电聚吡咯处理的膜最近已显示出在体外影响哺乳动物细胞的形态和功能。这种类型的聚合物代表了可能用于血管植入的替代生物材料。本研究比较了五种不同的聚酯机织织物的体外生物相容性，这些织物的电导率范围从4.5 x 10（4）到123 omega / square不断增加，与低密度聚乙烯和聚二甲基硅氧烷主要参考材料的体外生物相容性。根据四种不同类型的体外细胞反应来测量生物相容性，包括（a）间接和（b）使用内皮细胞的直接对照器官型培养测定，（c）使用流式细胞术的多形核（PMN）细胞激活研究测量CD11 / CD18整联蛋白分子表达，以及（d）使用逆转录酶聚合酶链反应对单核细胞/巨噬细胞上白介素（IL）-6 mRNA表达进行半定量。有机型培养研究表明，具有高电导率的织物表现出较低的细胞迁移，增殖和生存能力。来自10位健康成人供体的血液的PMN活化研究表明，两种导电性最高的织物能够识别反应性更高的供体。单核细胞/巨噬细胞的IL-6 mRNA表达水平随织物电导率水平的升高而降低。因此，本研究的结果表明，聚酯织物上的高电导率（<200Ω/平方）不利于内皮细胞的生长，迁移和生存。诱导升高的PMN激活;并影响单核细胞的细胞内代谢。他们还指出了电导率的特定范围（10（3）<10（4）Ω/平方），这与最佳的体外细胞反应有关。

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