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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Platelet adhesion onto wettability gradient surfaces in the absence and presence of plasma proteins.
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Platelet adhesion onto wettability gradient surfaces in the absence and presence of plasma proteins.

机译：在不存在和存在血浆蛋白的情况下，血小板粘附在润湿性梯度表面上。

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A wettability gradient was prepared on lowdensity polyethylene (PE) sheets by treating them in air with a corona from a knife-type electrode the power of which increased gradually along the sample length. The PE surfaces oxidized gradually with the increasing corona power and a wettability gradient was created on the surfaces, as evidenced by the measurement of water contact angles, Fourier transform infrared spectroscopy in the attenuated total reflectance mode, and electron spectroscopy for chemical analysis. The wettability gradient surfaces prepared were used to investigate the adhesion behavior of platelets in the absence and presence of plasma proteins in terms of the surface hydrophilicity/hydrophobicity of polymeric materials. The platelets adhered to the wettability gradient surfaces along the sample length were counted and examined by scanning electron microscopy (SEM). It was observed that the platelet adhesion in the absence of plasma proteins increased gradually as the surface wettability increased along the sample length. The platelets adhered to the hydrophilic positions of the gradient surface also were more activated (possessed more pseudo pods as examined by SEM) than on the more hydrophobic ones. However, platelet adhesion in the presence of plasma proteins decreased gradually with the increasing surface wettability; the platelets adhered to the surface also were more activated on the hydrophobic positions of the gradient surface. This result is closely related to plasma protein adsorption on the surface. Plasma protein adsorption on the wettability gradient surface increased with the increasing surface wettability. More plasma protein adsorption on the hydrophilic positions of the gradient surface caused less platelet adhesion, probably due to platelet adhesion inhibiting proteins, such as high-molecular-weight kininogen, which preferably adsorbs onto the surface by the so-called Vroman effect. It seems that both the presence of plasma proteins and surface wettability play important roles for platelet adhesion and activation.
机译：在低密度聚乙烯（PE）片上通过用刀状电极的电晕在空气中进行处理来制备可湿性梯度，其功率沿样品长度逐渐增加。随着水接触角的测量，衰减全反射模式下的傅立叶变换红外光谱法以及用于化学分析的电子光谱法的证明，PE表面随着电晕功率的增加而逐渐氧化，并在表面上形成了润湿性梯度。制备的润湿性梯度表面用于研究在不存在和存在血浆蛋白的情况下，根据聚合材料的表面亲水性/疏水性，血小板的粘附行为。计数沿着样品长度粘附在润湿性梯度表面上的血小板，并通过扫描电子显微镜（SEM）检查。观察到，在血浆蛋白不存在的情况下，血小板粘附力随着表面可湿性沿样品长度的增加而逐渐增加。粘附在梯度表面的亲水位置上的血小板也比疏水性更高的血小板活化（通过SEM检查具有更多的伪荚）。然而，存在血浆蛋白的血小板粘附力随着表面润湿性的增加而逐渐降低。粘附在表面上的血小板在梯度表面的疏水位置上也被激活。该结果与血浆蛋白在表面上的吸附密切相关。血浆蛋白在润湿性梯度表面的吸附随着表面润湿性的增加而增加。在梯度表面的亲水位置上更多的血浆蛋白吸附导致较少的血小板粘附，这可能是由于血小板粘附抑制蛋白，例如高分子量激肽原，其优选地通过所谓的弗罗曼效应吸附在表面上。血浆蛋白的存在和表面润湿性似乎对血小板粘附和活化起着重要作用。

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