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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Behavior of human osteoblast-like cells in contact with electrodeposited calcium phosphate coatings.
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Behavior of human osteoblast-like cells in contact with electrodeposited calcium phosphate coatings.

机译：人成骨样细胞与电沉积磷酸钙涂层接触的行为。

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摘要

Calcium-deficient hydroxyapatite (Ca-def-HAP) thin films were elaborated on Ti6Al4V substrates by electrodeposition. The coatings exhibit two different morphologies and crystallinities. Human osteoblast-like cells (MG-63) were cultured on the surfaces of these materials; the cell content and viability were evaluated up to 28 days. The scanning electron microscopy and biological investigations showed cells with a normal morphology, good proliferation, and viability from 7 to 21 days. But after 28 days, the number of live cells decreases in both cases; however, this decrease is less important in the case of calcium phosphate (CaP) coating surface when compared with the control (cell culture plastic). The cells cultured on Ca-def-HAP coating exhibit more cellular extensions and extracellular matrix. RT-PCR for type I collagen, alkaline phosphatase, and osteocalcin studies were also carried out, and was found that the CaP enhances gene expression of ALP and OC and thus the differentiation of osteoblast-like cells. Moreover, this study shows that the difference in the morphology of CaP coatings has no effect on the biocompatibility.
机译：通过电沉积法在Ti6Al4V基板上制备了钙缺乏的羟基磷灰石（Ca-def-HAP）薄膜。涂层表现出两种不同的形态和结晶度。在这些材料的表面上培养了人类成骨样细胞（MG-63）。评估细胞含量和生存力长达28天。扫描电子显微镜和生物学研究显示，细胞具有正常形态，良好的增殖能力和7到21天的生存能力。但是在28天后，两种情况下的活细胞数量都减少了。但是，与对照（细胞培养塑料）相比，这种减少对于磷酸钙（CaP）涂层表面而言不太重要。在Ca-def-HAP涂层上培养的细胞表现出更多的细胞延伸和细胞外基质。还进行了I型胶原，碱性磷酸酶和骨钙素的RT-PCR研究，发现CaP增强了ALP和OC的基因表达，从而促进了成骨细胞样细胞的分化。此外，这项研究表明，CaP涂层的形貌差异对生物相容性没有影响。

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