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首页> 外文期刊>Journal of bone and mineral research: the official journal of the American Society for Bone and Mineral Research >Isoforms of bone alkaline phosphatase: characterization and origin in human trabecular and cortical bone.
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Isoforms of bone alkaline phosphatase: characterization and origin in human trabecular and cortical bone.

机译：骨碱性磷酸酶的同工型：人小梁和皮质骨的特征和来源。

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摘要

Alkaline phosphatase (ALP) is a glycoprotein and functions as an ectoenzyme attached to the cell membrane by a hydrophobic glycosyl-phosphatidylinositol (GPI) anchor. Three bone ALP (BALP) isoforms in human serum were separated and quantitated by high-performance liquid chromatography. B/I, a minor fraction, is composed on average of bone (70%) and intestinal (30%) ALP, and two major isoforms, B1 and B2. Treatment with GPI-specific phospholipase C (GPI-PLC) did not influence the activities or retention times for B1 and B2, indicating that the biochemical differences between B1 and B2 are likely to be due to different glycosylation patterns. The B/I fraction in serum, on average 4% of total ALP, was found to be composed of B1 and B2 isoforms, each with an intact hydrophobic GPI cell membrane anchor. We investigated the origin of these three BALP isoforms and osteocalcin in human femora from five healthy individuals (four males), mean age 51 years, obtained from a tissue bank. Bone was sampled from three sites: cortical bone, trabecular bone from the diaphysis, and trabecular bone from the greater trochanter. Trabecular bone, from both sites, had higher BALP activities compared with cortical bone. Conversely, the osteocalcin content of cortical bone was more than 3-fold greater than that of trabecular bone. Cortical bone had approximately 2-fold higher activity of B1 compared with B2, whereas trabecular bone had approximately 2-fold higher activity of B2 compared with B1. We observed a previously undescribed BALP isoform (B1x) in all bone samples. B1x was also observed in sera from some patients (60%) with severe renal insufficiency and on chronic dialysis therapy (n = 20). The isoforms of BALP may provide information relating to bone metabolism within specific bone compartments.
机译：碱性磷酸酶（ALP）是一种糖蛋白，可作为通过疏水性糖基磷脂酰肌醇（GPI）锚连接到细胞膜的外切酶。通过高效液相色谱法分离并定量了人类血清中的三种骨ALP（BALP）亚型。 B / I是次要部分，平均由骨骼（70％）和肠道（30％）ALP以及两个主要同工型B1和B2组成。用GPI特异性磷脂酶C（GPI-PLC）处理不会影响B1和B2的活性或保留时间，这表明B1和B2之间的生化差异可能是由于糖基化方式不同所致。发现血清中的B / I分数平均占总ALP的4％，由B1和B2同工型组成，每个同工型均具有完整的疏水性GPI细胞膜锚。我们调查了来自组织库的五名健康人（四名男性）的平均年龄为51岁，这三种BALP亚型和骨钙素在人股骨中的起源。从三个位置取样骨骼：皮质骨，骨干骨的小梁骨和大转子的骨小梁。与皮质骨相比，两个部位的小梁骨都有更高的BALP活性。相反，皮质骨的骨钙素含量是小梁骨的3倍以上。皮质骨的B1活性比B2高约2倍，而小梁骨的B2活性比B1高约2倍。我们在所有骨样品中观察到先前未描述的BALP同工型（B1x）。在一些患有严重肾功能不全的患者（60％）的血清中也观察到了B1x（n = 20）。 BALP的同工型可以提供有关特定骨腔内骨代谢的信息。

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