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首页> 外文期刊>Journal of bone and mineral research: the official journal of the American Society for Bone and Mineral Research >Reduction of osteoclasts in a critical embryonic period is essential for inhibition of mouse tooth eruption.
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Reduction of osteoclasts in a critical embryonic period is essential for inhibition of mouse tooth eruption.

机译：在关键的胚胎时期减少破骨细胞对于抑制小鼠牙齿萌发至关重要。

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摘要

Alveolar bone resorption by osteoclasts is essential for tooth eruption. Osteoclast-deficient Csfm(op) homozygous (op/op) mice, which lack functional macrophage colony-stimulating factor (M-CSF), suffer from osteopetrosis and completely lack tooth eruption. Although osteoclasts appear, and osteopetrosis is cured with age in op/op mice, tooth eruption is never seen. This fact suggests that there is a critical period when osteoclasts are required for tooth eruption. In this study, to detect the critical period, we administered an antagonistic antibody directed against c-Fms, a receptor for M-CSF, to inbred C57BL/6 mice for various periods. Administration of this antibody decreased tartrate-resistant acid phosphatase-positive (TRAP) osteoclasts, and incisor eruption was completely inhibited by continual administration of this antibody from embryonic day 15.5 (E15.5) until postnatal day 12.5 (D12.5). A 1-day delay of this administration abolished the inhibition of incisor eruption. The number of TRAP-positive osteoclasts was significantly reduced between E16.5 and E18.5 in the mice treated with antibody from E15.5 compared with those treated from E16.5. These results indicate that this period, during which the number of osteoclasts decreases significantly, is critical for inhibiting incisor eruption in C57BL/6 mice.
机译：破骨细胞吸收牙槽骨对于牙齿萌发至关重要。缺少破骨细胞的Csfm（op）纯合子（op / op）小鼠缺乏功能性巨噬细胞集落刺激因子（M-CSF），患有骨质疏松症并且完全没有牙齿萌出。尽管在op / op小鼠中出现破骨细胞，并且随着年龄的增长，骨质沉着症得以治愈，但从未看到过牙齿萌出。这个事实表明，在需要一个破骨细胞来萌发牙齿的关键时期。在这项研究中，为了检测关键时期，我们向近交C57BL / 6小鼠施用了不同时期的针对c-Fms（M-CSF受体）的拮抗抗体。从胚胎第15.5天（E15.5）到产后第12.5天（D12.5）连续施用该抗体，可降低酒石酸耐药性酸性磷酸酶阳性（TRAP）破骨细胞的能力，并完全抑制了门齿萌发。给药1天的延迟取消了对门牙喷发的抑制。与用E16.5处理的小鼠相比，用E15.5抗体处理的小鼠的TRAP阳性破骨细胞数量在E16.5和E18.5之间显着减少。这些结果表明，在这段时间内破骨细胞的数量显着减少，对于抑制C57BL / 6小鼠的门牙萌发至关重要。

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