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首页> 外文期刊>Journal of bone and mineral research: the official journal of the American Society for Bone and Mineral Research >Identification, characterization, and isolation of a common progenitor for osteoclasts, macrophages, and dendritic cells from murine bone marrow and periphery
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Identification, characterization, and isolation of a common progenitor for osteoclasts, macrophages, and dendritic cells from murine bone marrow and periphery

机译：鉴定，鉴定和分离鼠骨髓和外周血中破骨细胞，巨噬细胞和树突状细胞的常见祖细胞

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Osteoclasts are specialized bone-resorbing cells that derive from monocyte precursors. We have identified three populations of cells with high osteoclastogenic potential in murine bone marrow, which expressed the phenotype B220-CD3-CD11b-/low CD115+ and either CD117hi, CD117intermediate, or CD117low. We have evaluated these populations for their ability to also generate macrophages and dendritic cells. At a single-cell level, the population expressing higher CD117 levels was able to generate bone-resorbing osteoclasts, phagocytic macrophages, and antigen-presenting dendritic cells in vitro with efficiencies of more than 90%, indicating that there exists a common developmental pathway for these cell types. Cells with osteoclastogenic potential also exist in blood and peripheral hematopoietic organs. Their functional meaning and/or their relationship with bone marrow progenitors is not well established. Hence, we characterized murine peripheral cell populations for their ability to form osteoclasts, macrophages, and dendritic cells in vitro. The spleen and peripheral blood monocyte progenitors share phenotypic markers with bone marrow progenitors but differ in their expression of CD11b, which was low in bone marrow but high in periphery. We propose that circulating monocyte progenitors are derived from a common bone marrow osteoclasts/ macrophage/dendritic cell progenitor (OcMDC), which we have now characterized at a clonal level. However, the lineage relationship between the bone marrow and peripheral monocyte progenitors has yet to be defined.
机译：破骨细胞是来源于单核细胞前体的专门的骨吸收细胞。我们已经确定了鼠骨髓中具有高破骨细胞潜力的三个细胞群，它们表达的表型为B220-CD3-CD11b- /低CD115 +和CD117hi，CD117intermediate或CD117low。我们评估了这些种群还产生巨噬细胞和树突状细胞的能力。在单细胞水平上，表达较高CD117水平的种群能够在体外产生吸收骨的破骨细胞，吞噬巨噬细胞和呈递抗原的树突状细胞，效率超过90％，这表明存在共同的发育途径这些细胞类型。具有破骨细胞潜能的细胞也存在于血液和外周造血器官中。它们的功能含义和/或它们与骨髓祖细胞的关系尚不十分清楚。因此，我们表征了鼠外周细胞群体在体外形成破骨细胞，巨噬细胞和树突状细胞的能力。脾脏和外周血单核细胞祖细胞与骨髓祖细胞具有表型标记，但CD11b的表达不同，CD11b在骨髓中较低，而在外周中较高。我们建议循环单核细胞祖细胞来自常见的骨髓破骨细胞/巨噬细胞/树突状细胞祖细胞（OcMDC），我们现在已经在克隆水平对其进行了表征。但是，骨髓与外周单核细胞祖细胞之间的谱系关系尚未确定。

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