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首页> 外文期刊>Journal of bone and mineral research: the official journal of the American Society for Bone and Mineral Research >Ultrastructural properties in cortical bone vary greatly in two inbred strains of mice as assessed by synchrotron light based micro- and nano-CT.
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Ultrastructural properties in cortical bone vary greatly in two inbred strains of mice as assessed by synchrotron light based micro- and nano-CT.

机译：两种近交系小鼠的皮质骨中超微结构特性的差异很大，这是通过基于同步加速器光的微型CT和纳米CT评估的。

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摘要

Nondestructive SR-based microCT and nano-CT methods have been designed for 3D quantification and morphometric analysis of ultrastructural phenotypes within murine cortical bone, namely the canal network and the osteocyte lacunar system. Results in two different mouse strains, C57BL/6J-Ghrhr(lit)/J and C3.B6-Ghrhr(lit)/J, showed that the cannular and lacunar morphometry and their bone mechanics were fundamentally different. INTRODUCTION: To describe the different aspects of bone quality, we followed a hierarchical approach and assessed bone tissue properties in different regimens of spatial resolution, beginning at the organ level and going down to cellular dimensions. For these purposes, we developed different synchrotron radiation (SR)-based CT methods to assess ultrastructural phenotypes of murine bone. MATERIALS AND METHODS: The femoral mid-diaphyses of 12 C57BL/6J-Ghrhr(lit)/J (B6-lit/lit) and 12 homozygous mutants C3.B6-Ghrhr(lit)/J (C3.B6-lit/lit) were measured with global SR microCT and local SRnano-CT (nCT) at nominal resolutions ranging from 3.5 microm to 700 nm, respectively. For volumetric quantification, morphometric indices were determined for the cortical bone, the canal network, and the osteocyte lacunar system using negative imaging. Moreover, the biomechanics of B6-lit/lit and C3.B6-lit/lit mice was determined by three-point bending. RESULTS: The femoral mid-diaphysis of C3.B6-lit/lit was larger compared with B6-lit/lit mice. On an ultrastructural level, the cannular indices for C3.B6-lit/lit were generally bigger in comparison with B6-lit/lit mice. Accordingly, we derived and showed a scaling rule, saying that overall cannular indices scaled with bone size, whereas indices describing basic elements of cannular and lacunar morphometry did not. Although in C3.B6-lit/lit, the mean canal volume was larger than in B6-lit/lit, canal number density was proportionally smaller in C3.B6-lit/lit, so that lacuna volume density was found to be constant and therefore independent of mouse strain and sex. The mechanical properties in C3.B6-lit/lit were generally improved compared with B6-lit/lit specimens. For C3.B6-lit/lit, we observed a sex specificity of the mechanical parameters, which could not be explained by bone morphometry on an organ level. However, there is evidence that for C3.B6-lit/lit, the larger cortical bone mass is counterbalanced or even outweighed by the larger canal network in the female mice. CONCLUSIONS: We established a strategy to subdivide murine intracortical porosity into ultrastructural phenotypes, namely the canal network and the osteocyte lacunar system. Nondestructive global and local SR-based CT methods have been designed for 3D quantification and subsequent morphometric analysis of these phenotypes. Results in the two different mouse strains C57BL/6J-Ghrhr(lit)/J and C3.B6-Ghrhr(lit)/J showed that the cannular and lacunar morphometry and the biomechanical properties were fundamentally different.
机译：已经设计了基于无损SR的microCT和nano-CT方法，用于鼠皮质骨（即管网和骨细胞腔系统）内超结构表型的3D定量和形态计量分析。在两种不同的小鼠品系C57BL / 6J-Ghrhr（lit）/ J和C3.B6-Ghrhr（lit）/ J中的结果表明，套管和腔隙的形态以及它们的骨骼力学是根本不同的。简介：为了描述骨骼质量的不同方面，我们采用了分级方法，并在不同的空间分辨率方案中评估了骨骼组织的特性，从器官水平开始一直到细胞尺度。为了这些目的，我们开发了不同的基于同步辐射（SR）的CT方法来评估鼠骨的超微结构表型。材料与方法：12个C57BL / 6J-Ghrhr（lit）/ J（B6-lit / lit）和12个纯合突变体C3.B6-Ghrhr（lit）/ J（C3.B6-lit / lit）的股骨中部phy骨）分别使用整体SR microCT和局部SRnano-CT（nCT）在标称分辨率范围为3.5微米至700 nm的范围内进行了测量。为了进行体积定量，使用负成像确定了皮质骨，管网和骨细胞腔系统的形态计量指标。此外，通过三点弯曲测定B6-lit / lit和C3.B6-lit / lit小鼠的生物力学。结果：C3.B6lit / lit的股骨中骨干比B6lit / lit的小鼠大。在超微结构水平上，C3.B6-lit / lit小鼠的肾小管指数通常比B6-lit / lit小鼠大。因此，我们推导并显示了缩放规则，即总的导管索引随骨骼大小缩放，而描述导管形态和腔隙形态基本要素的索引却没有。尽管在C3.B6-lit / lit中，平均管体积比B6-lit / lit中的大，但在C3.B6-lit / lit中，管的数量密度成比例地较小，因此发现腔隙体积密度是恒定的，并且因此独立于小鼠的品系和性别。与B6-lit / lit试样相比，C3.B6-lit / lit试样的机械性能通常得到改善。对于C3.B6-lit / lit，我们观察到了机械参数的性别特异性，这无法通过器官形态上的骨形态测量来解释。但是，有证据表明，对于C3.B6-lit / lit，雌性小鼠中较大的管网会抵消甚至大于其皮质骨质量。结论：我们建立了一种策略，将鼠皮质内孔隙度细分为超微结构表型，即管网和骨细胞腔系统。已经设计了基于无损全局和局部SR的CT方法，用于这些表型的3D定量和后续形态分析。两种不同的小鼠品系C57BL / 6J-Ghrhr（lit）/ J和C3.B6-Ghrhr（lit）/ J的结果表明，套管和腔隙的形态以及生物力学特性是根本不同的。

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