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首页> 外文期刊>Journal of bone and mineral research: the official journal of the American Society for Bone and Mineral Research >Bone healing and migration of cord blood-derived stem cells into a critical size femoral defect after xenotransplantation.
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Bone healing and migration of cord blood-derived stem cells into a critical size femoral defect after xenotransplantation.

机译：异种移植后，脐带血干细胞的骨愈合和迁移成为临界尺寸的股骨缺损。

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Stem cell and tissue engineering-based therapies have become a promising option to heal bony defects in the future. Human cord blood-derived mesenchymal stem cells were seeded onto a collagen/tricalcium phosphate scaffold and xenotransplanted into critical size femoral defects of 46 nude rats. We found a survival of human cells within the scaffold and surrounding bone/bone marrow up to 4 wk after transplantation and an increased bone healing rate compared with controls without stem cells. This study supports the application of cord blood stem cells for bone regeneration. INTRODUCTION: The treatment of critical size bone defects is still a challenging problem in orthopedics. In this study, the survival, migration, and bone healing promoting potency of cord blood-derived stem cells were elucidated after xenotransplantation into a critical size femoral defect in athymic nude rats. MATERIALS AND METHODS: Unrestricted somatic stem cells (USSCs) isolated from human cord blood were tested toward their mesenchymal in vitro potency and cultivated onto a collagen I/III and beta-tricalcium phosphate (beta-TCP) scaffold. The biomaterial-USSC composite was transplanted into a 4-mm femoral defect of 40 nude rats and stabilized by an external fixator. Twelve animals without USSCs served as controls. Cell survival, migration, and bone formation were evaluated by blood samples, X-rays, and histological and immunocytochemical analysis of different organs within a maximal postoperative follow-up of 10 wk. RESULTS: Of the 52 nude rats, 46 animals were evaluated (drop-out rate: 11.5%). Human-derived stem cells showed an engraftment within the scaffold and adjacent femur up to 4 wk after xenotransplantation. With further time, the human cells were destroyed by the host organism. We found a significant increase in bone formation in the study group compared with controls. USSC transplantation did not significantly influence blood count or body weight in athymic nude rats. Whereas the collagen I/III scaffold was almost resorbed 10 wk after transplantation, there were still significant amounts of TCP present in transplantation sites at this time. CONCLUSIONS: Human cord blood-derived stem cells showed significant engraftment in bone marrow, survived within a collagen-TCP scaffold up to 4 wk, and increased local bone formation in a nude rat's femoral defect.
机译：基于干细胞和组织工程的疗法已成为将来治愈骨缺损的有前途的选择。将人脐血来源的间充质干细胞接种到胶原蛋白/磷酸三钙支架上，并异种移植到46只裸鼠的临界尺寸股骨缺损中。我们发现，与没有干细胞的对照组相比，移植后支架和周围的骨/骨髓中人类细胞的存活率最高可达4 wk，并且骨愈合率提高。这项研究支持脐带血干细胞在骨骼再生中的应用。简介：关键尺寸骨缺损的治疗仍然是整形外科中的一个难题。在这项研究中，在无胸腺裸鼠异种移植到临界尺寸的股骨缺损后，阐明了脐带血干细胞的存活，迁移和骨愈合促进效能。材料与方法：从人脐带血中分离出的非限制性体细胞干细胞（USSCs）进行体外间充质测试，并培养在I / III胶原蛋白和β-磷酸三钙（β-TCP）支架上。这种生物材料-USSC复合材料被移植到40只裸鼠的4毫米股骨缺损中，并通过外固定器稳定。没有USSC的十二只动物作为对照。在术后最大随访10周内，通过血液样本，X射线以及不同器官的组织学和免疫细胞化学分析评估细胞的存活，迁移和骨形成。结果：在52只裸鼠中，评估了46只动物（辍学率：11.5％）。人源性干细胞在异种移植后长达4 wk的时间里都植入了支架和邻近股骨。随着时间的流逝，人类细胞被宿主生物破坏。我们发现与对照组相比，研究组的骨形成明显增加。 USSC移植并未显着影响无胸腺裸鼠的血球计数或体重。尽管胶原蛋白I / III支架在移植后10周几乎被吸收，但此时移植位点仍存在大量TCP。结论：人脐带血来源的干细胞显示出显着的骨髓移植，在胶原蛋白-TCP支架中存活至4周，并且在裸鼠股骨缺损中局部骨形成增加。

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