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首页> 外文期刊>Journal of Camel Practice and Research >LABORATORY AND FIELD INVESTIGATIONS OF A LIVE ATTENUATED AND AN INACTIVATED CAMELPOX VACCINE
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LABORATORY AND FIELD INVESTIGATIONS OF A LIVE ATTENUATED AND AN INACTIVATED CAMELPOX VACCINE

机译：现场和灭活骆驼痘疫苗的实验室和现场调查

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The aim of this work was to determine the efficacy of two commercially available camelpox (CP) vaccines, namely Ducapox live attenuated (LA) vaccine produced in South Africa and an inactivated adjuvant (IA) vaopine produced in Morocco. Inoculation ofthe LA vaccine virus into cell culture produced typical growth characteristics of a CP virus (CPV) with high virus titre. Sterility tests proved the safety of the vaccine. Young camels 12 tb 18 months of age were divided into groups and vaccinated with both vaccines with single and two doses regimes. Humoral immune response was measured by serum neutralisation tesl (SNT) and passive haemagglutination test (PHT). Cellular immune response was measured by the delayed type-hypersensitivity (DTH) reaction. Camels vaccinated with both vaccines and later challenged with a virulent CPV, showed no clinical signs of disease or pox lesions. Development of the humoral antibody response started on the 2nd week post vaccination (PV) for both vaccines and a booster dose increased the antibody titre significantly The IA vaccine, however, induced a low level of antibodies. In the DTH experiment, all camels vaccinated with both vaccines reacted positively with a remarkable increase in skin thickness as compared to controls indicating a sound cell-mediated immune (CMI) response The increase in thickness was relatively greater in dromedaries vaccinated with the LA than with the IA vaccine. Field vaccination trials revealed that both vaccines induced a weak immune response in camels less than 6 months of age and also older than/t years of age. This can be explained by possible existence of maternal antibodies or the immaturity of the immune system in camel calves, and the occurrence of pre-vaccination antibodies in adult camels. Good immune response as measured by seroiogical tests, developed in the age group of 1 to 4 year-old camels. Both vaccines used in this study were safe, potent and immunogenic "since they induced humoral and cellular immune response and protected vaccinated dromedaries against challenge. Camels vaccinated with the IA should be revaccinated 8 weeks post-primary vaccination, while one dose of the LA vaccine is enough to sustain protection for at least one year. The appropriate age for vaccination for both vaccines is 6 months. The Passive Haemagglutination Test was found useful for serology of CP; it is easy to perform, less expensive and also sensitive when compared with the serum neutralisation test.
机译：这项工作的目的是确定两种市售骆驼痘（CP）疫苗的功效，即南非生产的Ducapox减毒活疫苗（LA）和摩洛哥生产的灭活佐剂（IA）紫杉醇。将LA疫苗病毒接种到细胞培养物中产生具有高病毒滴度的CP病毒（CPV）的典型生长特征。无菌测试证明了疫苗的安全性。将18 tb月龄的12 tb的幼骆驼分为两组，分别接种单次和两次剂量的疫苗。体液免疫反应通过血清中和tesl（SNT）和被动血凝试验（PHT）进行测量。通过迟发型超敏反应（DTH）来测量细胞的免疫反应。骆驼接种了两种疫苗，后来又用强毒的CPV攻击，没有显示出疾病或痘病的临床迹象。两种疫苗均在接种后第二周（PV）开始产生体液抗体反应，加强剂量可显着提高抗体效价。然而，IA疫苗诱导的抗体水平较低。在DTH实验中，与对照相比，接种了两种疫苗的所有骆驼都发生了阳性反应，皮肤厚度显着增加，表明有良好的细胞介导的免疫（CMI）反应。接种LA的单峰骆驼的厚度增加相对较大。 IA疫苗。野外疫苗接种试验表明，两种疫苗均在小于6个月大且大于/ t岁的骆驼中诱导弱的免疫反应。这可能是由于骆驼犊牛中母源抗体的存在或免疫系统的不成熟以及成年骆驼中疫苗接种前抗体的出现而造成的。通过血清学测试测得的良好免疫反应是在1至4岁骆驼的年龄组中发展的。这项研究中使用的两种疫苗都是安全，有效和具有免疫原性的，“因为它们诱导了体液和细胞免疫反应并保护了接种的单峰骆驼免受攻击。接种过IA的骆驼应在初次接种疫苗后8周重新接种，而一剂LA疫苗足以维持至少一年的保护;两种疫苗的适当接种年龄均为6个月;被动血凝试验对CP的血清学检查很有用;与CT相比，它操作简便，价格便宜且敏感。血清中和试验。

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