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首页> 外文期刊>Journal of Cancer Research and Clinical Oncology >Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.
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Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.

机译：通过RT-PCR在61种人类肿瘤细胞系中进行全面的半乳凝素指纹图谱分析及其对诊断和治疗程序的意义。

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PURPOSE: Knowledge about galectin expression by human tumor cells is mainly restricted to galectins-1 and -3. This study was conducted to define the gene expression pattern of all presently known human galectins in tumor cell lines of various histogenetic origin (galectinomics). METHODS: The presence of mRNAs for human galectins-1, -2, -3, -4, -7, -8, and -9 was monitored by RT-PCR analyses in a panel of 61 human tumor cell lines of different origin (breast, colon, lung, brain, skin, kidney, urogenital system, hematopoietic system). RESULTS: The validity of the technique was first confirmed by comparison of RT-PCR data with those obtained by Western blotting and cytofluorometry for galectins-1 and -3 in 18 cell lines. The following detection of a complex pattern of gene expression beyond commonly studied galectins-1 and -3 underscored the need for this fingerprinting. The most abundantly expressed message for a member of this lectin family was galectin-8 with 59 positive cell lines. With the exception of the tested lung tumors, galectin-1 and -3 transcripts were frequently expressed in the cell line panel with differences between individual cases. Positivity for galectins-2 and -4 was confined to a significant fraction of colorectal and neural tumors. Signals for galectin-9, the third known human tandem-repeat-type galectin besides -4 and -8, appeared in colorectal carcinoma cell lines with a frequency similar to that of galectin-4 but with inter-line differences. Its expression was restricted to lines of this tumor type, of the tested ovarian carcinoma, and hematopoietic malignancies. CONCLUSIONS: The results clearly demonstrate that human tumor cells express more mRNA species for galectins than those for galectins-1 and -3. To derive unequivocal diagnostic and prognostic information by immunohistochemistry on galectins with antagonistic impact on growth control and significant influence on cell adhesion, additional monitoring of these so far insufficiently studied family members is essential.
机译：目的：关于人类肿瘤细胞表达半乳凝素的知识主要限于半乳凝素-1和-3。进行了这项研究以定义各种组织遗传学起源的肿瘤细胞系中所有目前已知的人半乳凝素的基因表达模式（半乳组学）。方法：通过RT-PCR分析在一组61种不同来源的人类肿瘤细胞系中检测人类半乳糖凝集素-1，-2，-3，-4，-7，-8和-9 mRNA的存在（乳房，结肠，肺，脑，皮肤，肾脏，泌尿生殖系统，造血系统）。结果：该技术的有效性首先通过将RT-PCR数据与18个细胞系中Galectins-1和-3的Western blotting和细胞荧光检测的数据进行比较来证实。除了常规研究的半乳糖凝集素-1和-3之外，随后检测到一种复杂的基因表达模式，突显了这种指纹识别的必要性。该凝集素家族成员中表达最丰富的信息是具有59个阳性细胞系的半乳凝素-8。除了所测试的肺肿瘤外，galectin-1和-3转录本经常在细胞系中表达，各病例之间存在差异。 galectins-2和-4的阳性率仅限于大部分的结直肠和神经肿瘤。 galectin-9的信号是除-4和-8以外的第三个已知的人串联重复型galectin，其信号以与galectin-4相似的频率出现在结直肠癌细胞系中，但存在系间差异。它的表达仅限于这种类型的肿瘤，卵巢癌和造血系统恶性肿瘤。结论：结果清楚地表明，人肿瘤细胞表达的半乳糖凝集素比半乳糖凝集素-1和-3更多。为了通过半组织凝集素的免疫组织化学方法获得明确的诊断和预后信息，从而对生长控制产生不利影响，并对细胞粘附产生显着影响，因此对这些迄今研究不足的家族成员进行额外的监测是必不可少的。

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