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首页> 外文期刊>Journal of Cancer Research and Clinical Oncology >A pilot study comparing protein expression in different segments of the normal colon and rectum and in normal colon versus adenoma in patients with Lynch syndrome
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A pilot study comparing protein expression in different segments of the normal colon and rectum and in normal colon versus adenoma in patients with Lynch syndrome

机译：一项初步研究比较了Lynch综合征患者正常结肠和直肠不同部分以及正常结肠与腺瘤中蛋白质的表达

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Purpose: Lynch syndrome (LS) is a common inherited predisposition to colorectal cancer (CRC). In LS patients, CRC is predominantly located in the right colon, as opposed to sporadic CRC, which usually affects the left colon or rectum. Previous studies have demonstrated a clear distinction in gene expression between sporadic CRC and normal colon at different locations in the colorectum. However, little is known about LS gene expression profiles in different areas of the colorectum. Here, we compared the protein expression profiles for normal colorectal samples among different locations as well as between adenomas and matched normal tissue in LS. Methods: Protein from 33 tissue samples (27 normal tissues and 6 adenomas) from 9 patients with LS was extracted for reverse-phase protein array (RPPA) analysis. The antibody panel used for RPPA included 109 key proteins involved in various cancer-related pathways. Cluster 3.0 was used for unsupervised and supervised clustering analysis. Results: IGF1R and COL6A1 were expressed significantly differently between the normal right and normal left colon (q < 0.05); FN1, COL6A1, and IGF1R were expressed significantly differently between the normal right colon and normal rectum (q < 0.05). In the adenomas and matched normal tissue, PEA-15 was the only protein with significantly different expression (q < 0.05). Conclusion: We found differences in protein expression between normal tissues from the right colon, left colon, and rectum as well as between adenomas and matched normal tissue. However, those differences should be further confirmed in a larger sample size.
机译：目的：林奇综合征（LS）是大肠癌（CRC）的常见遗传易感性。在LS患者中，CRC主要位于右结肠，而散发性CRC通常会影响左结肠或直肠。先前的研究表明散发性CRC与结直肠不同部位正常结肠之间的基因表达存在明显区别。然而，关于结肠直肠不同区域中LS基因表达谱的了解很少。在这里，我们比较了正常结肠直肠样品在不同位置之间以及腺瘤和LS中匹配的正常组织之间的蛋白质表达谱。方法：提取9例LS患者的33个组织样本（27个正常组织和6个腺瘤）中的蛋白质，进行反相蛋白质阵列（RPPA）分析。用于RPPA的抗体组包括109种与各种癌症相关途径有关的关键蛋白。将Cluster 3.0用于无监督和有监督的聚类分析。结果：正常的右结肠和正常的左结肠之间IGF1R和COL6A1的表达显着不同（q <0.05）; FN1，COL6A1和IGF1R在正常右结肠和正常直肠之间表达差异显着（q <0.05）。在腺瘤和匹配的正常组织中，PEA-15是唯一表达差异显着的蛋白质（q <0.05）。结论：我们发现右结肠，左结肠和直肠的正常组织之间以及腺瘤和匹配的正常组织之间的蛋白质表达存在差异。但是，应该以更大的样本量进一步确认这些差异。

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