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首页> 外文期刊>Journal of Biophotonics >Multiphoton microscopy system with a compact fiber-based femtosecond-pulse laser and handheld probe
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Multiphoton microscopy system with a compact fiber-based femtosecond-pulse laser and handheld probe

机译：多光子显微镜系统，带有紧凑的基于光纤的飞秒脉冲激光和手持式探头

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摘要

We report on the development of a compact multiphoton microscopy (MPM) system that integrates a compact and robust fiber laser with a miniature probe. The all normal dispersion fiber femtosecond laser has a central wavelength of 1.06 μm, pulse width of 125 fs and average power of more than 1 W. A double cladding photonic crystal fiber was used to deliver the excitation beam and to collect the two-photon signal. The hand-held probe included galvanometer-based mirror scanners, relay lenses and a focusing lens. The packaged probe had a diameter of 16 mm. Second harmonic generation (SHG) images and two-photon excited fluorescence (TPEF) images of biological tissues were demonstrated using the system.
机译：我们报告了紧凑型多光子显微镜（MPM）系统的开发情况，该系统将紧凑而坚固的光纤激光器与微型探头集成在一起。全普通色散光纤飞秒激光器的中心波长为1.06μm，脉冲宽度为125 fs，平均功率大于1W。使用双包层光子晶体光纤传输激发光束并收集双光子信号。手持式探头包括基于振镜的反射镜扫描仪，中继透镜和聚焦透镜。包装的探针的直径为16mm。使用该系统演示了生物组织的二次谐波生成（SHG）图像和双光子激发荧光（TPEF）图像。

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