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首页> 外文期刊>Journal of Cerebral Blood Flow and Metabolism: Official Journal of the International Society of Cerebral Blood Flow and Metabolism >Micro-heterogeneity of flow in a mouse model of chronic cerebral hypoperfusion revealed by longitudinal Doppler optical coherence tomography and angiography
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Micro-heterogeneity of flow in a mouse model of chronic cerebral hypoperfusion revealed by longitudinal Doppler optical coherence tomography and angiography

机译：纵向多普勒光学相干断层扫描和血管造影显示的慢性脑低灌注小鼠模型中血流的微观异质性

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Although microvascular dysfunction accompanies cognitive decline in aging, vascular dementia, and Alzheimer's disease, tools to study microvasculature longitudinally in vivo are lacking. Here, we use Doppler optical coherence tomography (OCT) and angiography for noninvasive, longitudinal imaging of mice with chronic cerebral hypoperfusion for up to 1 month. In particular, we optimized the OCT angiography method to selectively image red blood cell (RBC)-perfused capillaries, leading to a novel way of assessing capillary supply heterogeneity in vivo. After bilateral common carotid artery stenosis (BCAS), cortical blood flow measured by Doppler OCT dropped to half of baseline throughout the imaged tissue acutely. Microscopic imaging of the capillary bed with OCT angiography further revealed local heterogeneities in cortical flow supply during hypoperfusion. The number of RBC-perfused capillaries decreased, leading to increased oxygen diffusion distances in the days immediately after BCAS. Linear regression showed that RBC-perfused capillary density declined by 0.3% for a drop in flow of 1 mL/100 g per minute, and decreases in RBC-perfused capillary density as high as 25% were observed. Taken together, these results demonstrate the existence of local supply heterogeneity at the capillary level even at nonischemic global flow levels, and demonstrate a novel imaging method to assess this heterogeneity.
机译：尽管微血管功能障碍伴随着衰老，血管性痴呆和阿尔茨海默氏病的认知下降，但缺乏在体内纵向研究微脉管系统的工具。在这里，我们使用多普勒光学相干断层扫描（OCT）和血管造影对慢性脑灌注不足的小鼠进行长达1个月的无创纵向成像。特别是，我们优化了OCT血管造影方法，以选择性地对灌注了红细胞（RBC）的毛细管成像，从而获得了一种评估体内毛细管供应异质性的新方法。在双侧颈总动脉狭窄（BCAS）后，通过多普勒OCT测量的皮层血流量在整个成像组织中急剧下降至基线的一半。用OCT血管造影术对毛细管床进行的显微成像进一步揭示了灌注不足时皮质血流供应的局部异质性。灌注RBC的毛细血管数量减少，导致在BCAS之后的几天内氧气扩散距离增加。线性回归显示，每分钟流量降低1 mL / 100 g，RBC灌注的毛细管密度下降了0.3％，RBC灌注的毛细管密度下降了25％。综上所述，这些结果证明了即使在非局部缺血的整体血流水平，在毛细管水平上也存在局部供应异质性，并证明了评估这种异质性的新颖成像方法。

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