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Screen-printed biosensor for allergens

机译：丝网印刷的过敏原生物传感器

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Allergen levels in indoor environments, leading to many diseases, eg asthma, rhinitis and conjunctivitis, affect a large and increasing fraction of the population. A quite effective and inexpensive method of a rough but very rapid overall assessment of total allergen level in the environment has been developed. The method involved estimation of protein in allergen extracts by screen-printed electrodes using two different techniques. The biosensor comprised a rhodinised carbon working electrode, a silver/silver chloride reference electrode and a carbon counter electrode. In the first method the enzyme protease reacted with allergen protein to release amino acid, which produced hydrogen peroxide in the presence of amino acid oxidase. This was detected amperometrically. The second method used potassium bromide as electrolyte and the electrode was subjected to dual potential. Bromine, released due to electrolysis at higher potential, was consumed by the allergen protein at lower potential. In the first method, a unique technique was used to microencapsulate the enzyme protease and immobilise it on the surface of the electrode by in-situ polymerisation to avoid contact with the amino acid oxidase. A total of seven allergens were tested and the results gave a good correlation with the standard protein measurement method. Environmental specimens from indoors, schools and workplaces can be evaluated for the aeroallergens produced by dust mites, animal hairs, cockroach debris, pollens, etc as a means of determining the exposure risk. (c) 2005 Society of Chemical Industry.
机译：室内环境中的过敏原水平会导致许多疾病，例如哮喘，鼻炎和结膜炎，影响着人口的不断增加。已经开发出一种非常有效和廉价的方法，可以对环境中的总过敏原水平进行粗略但非常快速的总体评估。该方法涉及使用两种不同技术通过丝网印刷电极估算过敏原提取物中的蛋白质。该生物传感器包括一个镀铑的碳工作电极，一个银/氯化银参比电极和一个碳反电极。在第一种方法中，酶蛋白酶与变应原蛋白反应释放出氨基酸，在氨基酸氧化酶存在下会产生过氧化氢。这是通过电流分析法检测到的。第二种方法使用溴化钾作为电解质，并使电极处于双电势。由于较高电位的电解而释放的溴被较低电位的过敏原蛋白消耗。在第一种方法中，采用一种独特的技术将酶蛋白酶微囊化，并通过原位聚合将其固定在电极表面上，以避免与氨基酸氧化酶接触。总共测试了七种过敏原，结果与标准蛋白质测量方法具有良好的相关性。可以评估室内，学校和工作场所的环境标本中的尘螨，动物毛，蟑螂碎片，花粉等所产生的气敏性物质，以此来确定接触风险。 （c）2005年化学工业协会。

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