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首页> 外文期刊>Journal of Chemical Technology & Biotechnology >The quest for alternatives to microbial cellulase mix production: Corn stover-produced heterologous multi-cellulases readily deconstruct lignocellulosic biomass into fermentable sugars
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The quest for alternatives to microbial cellulase mix production: Corn stover-produced heterologous multi-cellulases readily deconstruct lignocellulosic biomass into fermentable sugars

机译：寻求替代微生物纤维素酶混合物的生产方式：玉米秸秆生产的异源多纤维素酶很容易将木质纤维素生物质分解为可发酵糖

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BACKGROUND: Production of cellulosic ethanol is still expensive compared with corn (maize) grain ethanol due to the high costs of bulk production of microbial cellulases. At least three cellulases including endo-cellulase, exo-cellulase and cellobiase are needed to convert cellulosic biomass into fermentable sugars. All these cellulases could be self-produced within cells of transgenic bio-energy crops. The production of heterologous Acidothermus cellulolyticus (E1) endo-cellulase in endoplasmic reticulum and mitochondria of green tissues of transgenic corn plants was recently reported, and it was confirmed that the heterologous E1 converts cellulose into fermentable sugars. RESULTS: Biologically active A. cellulolyticus E1, Trichoderma reesei 1,4-β-cellobiohydrolases I (CBH I) exo-cellulase and bovine rumen Butyrivibrio fibrisolvens cellobiase were expressed in corn plant endoplasmic reticulum (ER), apoplast (cell wall areas) and vacuole respectively. Results show that the ratio 1:4:1 (E1:CBH I:cellobiase) of crude heterologous cellulases is ideal for converting ammonia fiber explosion (AFEX) pretreated corn stover into fermentable sugars. CONCLUSIONS: Corn plants that express all three biologically active heterologous cellulases within their cellulosic biomass to facilitate conversion of pretreated corn stover into fermentable sugars is a step forward in the quest for alternatives to the present microbial cellulase mix production for cellulosic biofuels.
机译：背景：由于大量生产微生物纤维素酶，与玉米（玉米）乙醇相比，纤维素乙醇的生产仍然昂贵。需要至少三种纤维素酶，包括内切纤维素酶，外切纤维素酶和纤维二糖酶，以将纤维素生物质转化为可发酵糖。所有这些纤维素酶都可以在转基因生物能源作物的细胞中自我产生。最近报道了在转基因玉米植物的绿色组织的内质网和线粒体中异源嗜酸解酸酶（E1）内切纤维素酶的产生，并且证实了异源E1将纤维素转化为可发酵的糖。结果：具有生物活性的A. cellulolyticus E1，里氏木霉1,4-β-纤维二糖水解酶I（CBH I）外切纤维素酶和牛瘤胃Butyrivibrio纤溶酶纤维二糖酶在玉米植物内质网（ER），质外体（细胞壁区域）和玉米中表达空泡分别。结果表明，粗异源纤维素酶的比例为1：4：1（E1：CBH I：纤维二糖酶）是将氨纤维爆炸（AFEX）预处理的玉米秸秆转化为可发酵糖的理想选择。结论：在纤维素生物质中表达所有三种具有生物活性的异质纤维素酶以促进将预处理的玉米秸秆转化为可发酵糖的玉米植物，是寻找替代目前用于纤维素生物燃料的微生物纤维素酶混合物的替代品的一步。

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