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首页> 外文期刊>Journal of Chemical Technology & Biotechnology >Optimization of growth operational conditions for CO_2 biofixation by native Synechocystis sp.
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Optimization of growth operational conditions for CO_2 biofixation by native Synechocystis sp.

机译：天然Synechocystis sp。的CO_2生物固定生长操作条件的优化。

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BACKGROUND: A fundamental step in assessing the viability of a CO_2 biofixation system based on microalgae is to identify the maximum CO_2 biofixation yield that can be achieved for this microorganism when it is cultivated under optimum operational growth conditions. Response surface methodology was applied to determine optimum culture conditions for CO_2 biofixation by a recently isolated freshwater cyanobacterium Synechocystis sp. The strain was cultivated in a 1 L bubble column photobioreactor, in semicontinuous mode. RESULTS: Statistical analysis showed that temperature (from 22 to 39 °C), pH (from 7.2 to 8.8) and light intensity (from 928 to 2272 μE m~(-2) s~(-1)), in addition to some of their interactions, had a significant effect on CO_2 biofixation. An optimum CO_2 biofixation rate of 2.07 gCO_2 L~(-1)culture day~(-1) was found within the experimental region, at an average light intensity 686 μE m-2 s-1, pH 7.2 and temperature 35.3 °C. CONCLUSIONS: Based on these results, it is concluded that Synechocystis sp. presents a good tolerance to both high temperature and light intensity, characteristics which facilitate its application in outdoor CO_2 biofixation systems.
机译：背景：评估基于微藻的CO_2生物固定系统的可行性的基本步骤是，确定在最佳操作生长条件下培养该微生物可实现的最大CO_2生物固定产量。应用响应面方法确定了最近分离的淡水蓝藻蓝藻属细菌的最佳CO_2生物固定培养条件。该菌株在1 L鼓泡塔光生物反应器中以半连续模式培养。结果：统计分析表明，温度（22至39°C），pH（7.2至8.8）和光强度（928至2272μEm〜（-2）s〜（-1））除外。他们的相互作用，对CO_2生物固定有重大影响。在平均光强度为686μEm-2 s-1，pH值为7.2和温度为35.3°C的条件下，实验区域内的最佳CO_2生物固定率为2.07 gCO_2 L〜（-1）培养日〜（-1）。结论：基于这些结果，得出结论：集胞藻属。对高温和光强度都表现出良好的耐受性，这有利于其在室外CO_2生物固定系统中的应用。

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