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首页> 外文期刊>Journal of Chemical Technology & Biotechnology >Optimization of cordycepin extraction from cultured Cordyceps militaris by HPLC-DAD coupled with uniform design
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Optimization of cordycepin extraction from cultured Cordyceps militaris by HPLC-DAD coupled with uniform design

机译：HPLC-DAD结合均匀设计优化虫草中虫草素的提取工艺

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BACKGROUND: Uniform design was used to optimize the ultrasonic-assisted extraction of cordycepin from cultured Cordyceps militaris. The peak area of cordycepin identified using high performance liquid chromatography (HPLC) at a detection wavelength of 260 mn was considered the detection index. Three factors, ethanol concentration, extraction time and volume ratio of solvent to sample were studied. Optimal quadric polynomial step by step regression was applied to process the experimental results. RESULTS: Results show that the main factors affecting cordycepin extraction yield were the volume ratio of solvent to sample, extraction time and ethanol concentration, in that order. Cordycepin extraction yield reached a peak with ethanol concentration 20.21%, extraction time 101.88min, and volume ratio of solvent to sample 33.13 mL g(-1). An extraction model was developed based on the findings. CONCLUSION: A direct, reliable and accurate assay has been developed for the quantification of cordycepin in cultured Cordyceps militaris by a HPLC-DAD method. The validation procedure confirmed that this method is appropriate for the quality control of cordycepin. Results show that the uniform experimental design approach is useful for finding polynomial functions describing the relationships between variables and responses and to find the best experimental conditions for the extraction of cordycepin in the experimental domain considered. (c) 2007 Society of Chemical Industry.
机译：背景：统一设计用于优化超声波法从培养的culture虫草中提取虫草素。使用高效液相色谱（HPLC）在260 mn的检测波长处鉴定到的虫草素的峰面积被认为是检测指标。研究了乙醇浓度，提取时间和溶剂与样品体积比这三个因素。应用最优二次多项式逐步回归来处理实验结果。结果：结果表明，影响虫草素提取率的主要因素依次是溶剂与样品的体积比，提取时间和乙醇浓度。虫草素的提取得率最高，乙醇浓度为20.21％，提取时间为101.88min，溶剂与样品的体积比为33.13 mL g（-1）。基于这些发现开发了提取模型。结论：已经开发了一种直接，可靠和准确的测定方法，用于通过HPLC-DAD方法定量测定Cord虫草中虫草素的含量。验证程序确认该方法适用于虫草素的质量控制。结果表明，统一的实验设计方法可用于找到描述变量与响应之间关系的多项式函数，并在考虑的实验范围内找到提取虫草素的最佳实验条件。 （c）2007年化学工业协会。

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