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首页> 外文期刊>Journal of Chemical Technology & Biotechnology >On the improvement of bacterial growth on complementary substrates by partial segregration in the broth
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On the improvement of bacterial growth on complementary substrates by partial segregration in the broth

机译：通过肉汤中的部分分离改善互补基质上细菌的生长

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Previous studies have shown that when two species compete for the same substrate, a controlled degree of segregation in a bioreactor favours growth of the desired species. That concept has been extended to the growth of bacterial on a mixture of two substrates for one of which the organism has a natural preference. However, using the example of Escherichia coli cultivated in a medium containing glucose and fumarate, it has been shown that, while the physiological preference for glucose is maintained, incomplete macromixing promotes growth and enables better utilisation of both substrates. While the preferred substrate(glucose) is consumed first in a perfeetly mixed reactor, imperfect mixing allows both substrates to be utillised simultaneously, at different rates. By dividing the broth conceptually into an inoculated region and a non-inoculated region, it is seen that cell growth is favoured by an initial period of good mixing followed by poor mixing in the non-inoculated region.
机译：先前的研究表明，当两个物种竞争同一底物时，生物反应器中受控的偏析程度有利于所需物种的生长。该概念已扩展到细菌在两种底物的混合物上的生长，其中一种是生物自然优先选择的。然而，以在包含葡萄糖和富马酸酯的培养基中培养的大肠杆菌为例，已经表明，尽管维持了对葡萄糖的生理学偏好，但是不完全的宏观混合促进了生长并且能够更好地利用两种底物。虽然优选的底物（葡萄糖）首先在充分混合的反应器中消耗，但不完全混合可使两种底物以不同的速率同时使用。通过将肉汤从概念上划分为接种区和非接种区，可以看出，良好混合的初始阶段有利于细胞生长，然后在非接种区混合不良。

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