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首页> 外文期刊>Journal of Chemical Technology & Biotechnology >Dye bleaching and phenol precipitation by phthalic anhydride-modified horseradish peroxidase
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Dye bleaching and phenol precipitation by phthalic anhydride-modified horseradish peroxidase

机译：邻苯二甲酸酐改性的辣根过氧化物酶对染料的漂白和苯酚的沉淀

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摘要

The phenol precipitation and dye bleaching capabilities of phthalic anhydride-modified horseradish peroxidase C (PA-HRP) were compared with those of native HRP C and ethylene glycol-bis-(succinic acid N-hydroxysuccinimide ester)-modified URP (EG-HRP) reported previously. Removal efficiency (percentage of phenol removed from solution under experimental conditions) was determined for native HRP and both modified forms. Removal efficiencies at 37 “C were very similar, with >95% removal in each case. Removal efficiencies were less at 70 deg C overall (range 25-45%) bQt PA-and EG-HRP removed up to 50% more phenol than native HRP. The three HRP forms showed similar dye bleaching performance at 37deg C in the presence of H202 and accelerators (up to 86% colour removal). PA-and EG-HRP showed slightly greater bleaching abilities at 65 “C than native HRP for some of the dyelaccelerator combinations tested. Modified HRPs performed better in 40% (vlv) mixtures of dioxane or dimethylformamide.
机译：比较了邻苯二甲酸酐改性的辣根过氧化物酶C（PA-HRP）与天然HRP C和乙二醇-双-（琥珀酸N-羟基琥珀酰亚胺酯）改性的URP（EG-HRP）的苯酚沉淀和染料漂白能力以前报道过。测定天然HRP和两种修饰形式的去除效率（在实验条件下从溶液中去除的苯酚百分比）。在37℃下的去除效率非常相似，每种情况下去除率均> 95％。总体上在70摄氏度（25-45％范围）下，去除效率较低，与天然HRP相比，bQt PA-和EG-HRP去除的苯酚最多高出50％。在H2O2和促进剂存在下，三种HRP形式在37°C时显示出相似的染料漂白性能（高达86％的脱色率）。对于某些测试的染料加速剂组合，PA-和EG-HRP在65℃下的漂白能力比天然HRP略强。改性的HRP在二恶烷或二甲基甲酰胺的40％（vlv）混合物中表现更好。

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