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首页> 外文期刊>WIS:Wheat Information Service >Variation in genotypic responses of Indian hexaploid wheats for haploid production in crosses with maize
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Variation in genotypic responses of Indian hexaploid wheats for haploid production in crosses with maize

机译：印度六倍体小麦与玉米杂交产生单倍体的基因型反应的变异

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摘要

To study genotypic effects of wheat plant on haploid production through wheat x maize crossing, five F_1 hybrids of wheat (W_1--W_5) obtained from crossing of different hexaploid wheat varieties were pollinated with pollen from a composite variety ofmaize Kanchan in outdoor conditions. Cut glume method was used for emasculation. Hormonal treatment was 75 ppm solution of 2,4-D for two days after pollination and 300 ppm solution of gibberellic acid on the 3rd day. Seed set, rates of embryo formation,embryo germination and plantiet recovery were studied. Seed set was not directly affected by wheat genotypes. Response of wheat genotypes was different for embryo formation rate. The best genotype was W_1 with 12.8% embryo formation, while the poorest genotype W2 showed 2.5% embryo formation. The pollen tube growth measurements inside the stigma conducted on W_1 and W_5 crosses showed a significant difference; W_1 had a higher mean of pollen tube length than W_5, which was in agreement with their embryoproduction percentage. It was suggested that growth of maize pollen tube inside the stigma of wheat genotypes followed by embryo production might be under control by some unknown crossability systems different from that by Kr genes. No genotypic effectwas observed for embryo germination and plantiet recovery.
机译：为了研究小麦植物对小麦x玉米杂交对单倍体生产的基因型影响，在室外条件下，用来自不同六倍体小麦品种杂交的5个F_1小麦杂种（W_1--W_5）进行花粉授粉。割胶法用于去雄。激素处理是授粉后两天使用75 ppm的2,4-D溶液进行授粉，第三天使用300 ppm的赤霉素溶液进行激素处理。研究了种子结实，胚形成率，胚萌发和植株恢复。种子结实不受小麦基因型的直接影响。小麦基因型对胚形成率的反应不同。最佳基因型为W_1，胚胎形成率为12.8％，而最差的基因型W2为2.5％胚胎形成。在W_1和W_5杂交的柱头内部的花粉管生长测量结果显示出显着差异。 W_1的花粉管平均长度高于W_5，这与它们的胚胎产生百分比一致。有人认为，玉米花粉管在小麦基因型柱头内部的生长以及随后的胚胎生产可能受到一些与Kr基因不同的未知的可交叉性系统的控制。没有观察到胚胎发芽和植物恢复的基因型效应。

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