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首页> 外文期刊>Journal of Cell Science >Compartmentalization of the functions and regulation of the mitotic cyclin Clb2 in S. cerevisiae.
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Compartmentalization of the functions and regulation of the mitotic cyclin Clb2 in S. cerevisiae.

机译：分隔的功能和调控有丝分裂细胞周期蛋白Clb2在酿酒酵母中。

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摘要

Orderly progression through the eukaryotic cell cycle is a complex process involving both regulation of cyclin dependent kinase activity and control of specific substrate-Cdk interactions. In Saccharomyces cerevisiae, the mitotic cyclin Clb2 has a central role in regulating the onset of anaphase and in maintaining the cellular shape of the bud by inhibiting growth polarization induced in G1. However, how Clb2 and the partially redundant cyclin Clb1 confer specificity to Cdk1 in these processes still remains unclear. Here, we show that Clb2 mutants impaired in nuclear import or export are differentially affected for subsets of Clb2 functions while remaining fully functional for others. Our data support a direct role of the cytoplasmic pool of Clb1,2-Cdk1 in terminating cytoskeleton and growth polarization, independently of G1 cyclin transcriptional regulation. By contrast, the nuclear form of the cyclin is required for timely initiation of anaphase. Clb2 localization influences its stage-specific degradation as well. We report that Clb2 trapped in the cytoplasm is stabilized during anaphase but not at the time of mitotic exit. Altogether, our results demonstrate that the subcellular localization of the mitotic cyclin Clb2 is one of the key determinants of its biological function.
机译：通过真核细胞周期的有序进展是一个复杂的过程，涉及调节细胞周期蛋白依赖性激酶活性和控制特定底物-Cdk相互作用。在酿酒酵母中，有丝分裂细胞周期蛋白Clb2在调节后期发育中起主要作用，并通过抑制G1诱导的生长极化来维持芽的细胞形状。但是，在这些过程中，Clb2和部分冗余的细胞周期蛋白Clb1如何赋予Cdk1特异性仍不清楚。在这里，我们显示在核输入或输出中受损的Clb2突变体对Clb2功能的子集有不同的影响，而对其他功能则保持完全功能。我们的数据支持Clb1,2-Cdk1的细胞质池在终止细胞骨架和生长极化中的直接作用，而与G1细胞周期蛋白的转录调控无关。相比之下，细胞周期蛋白的核形式是及时开始后期所需的。 Clb2的定位也会影响其特定阶段的降解。我们报告说，捕获在细胞质中的Clb2在后期稳定，但在有丝分裂退出时却不稳定。总之，我们的结果表明，有丝分裂细胞周期蛋白Clb2的亚细胞定位是其生物学功能的关键决定因素之一。

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