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首页> 外文期刊>Journal of Cell Science >Cajal body proteins SMN and Coilin show differential dynamic behaviour in vivo.
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Cajal body proteins SMN and Coilin show differential dynamic behaviour in vivo.

机译：卡哈尔体蛋白SMN和Coilin在体内表现出不同的动态行为。

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摘要

Analysis of stable cell lines expressing fluorescently tagged survival of motor neurons protein (SMN) and coilin shows striking differences in their dynamic behaviour, both in the nucleus and during mitosis. Cajal bodies labelled with either FP-SMN or FP-coilin show similar behaviour and frequency of movements. However, fluorescence recovery after photobleaching (FRAP) studies show that SMN returns approximately 50-fold more slowly to Cajal bodies than does coilin. Time-lapse studies on cells progressing from prophase through to G1 show further differences between SMN and coilin, both in their localisation in telophase and in the timing of their re-entry into daughter nuclei. The data reveal similarities between Cajal bodies and nucleoli in their behaviour during mitosis. This in vivo study indicates that SMN and coilin interact differentially with Cajal bodies and reveals parallels in the pathway for reassembly of nucleoli and Cajal bodies following mitosis.
机译：对表达荧光标记的运动神经元蛋白（SMN）和卷曲蛋白存活的稳定细胞系的分析显示，在细胞核和有丝分裂期间，它们的动态行为都存在显着差异。标有FP-SMN或FP-coilin的卡哈尔体显示出相似的行为和移动频率。然而，光漂白后的荧光恢复（FRAP）研究表明，SMN向Cajal体的返回比卷取蛋白慢约50倍。对从前期细胞发展到G1细胞的延时研究表明，SMN和线圈蛋白之间存在进一步的差异，既位于末期，又位于重新进入子核的时间。数据揭示了Cajal体和核仁在有丝分裂期间的行为相似。这项体内研究表明，SMN和线圈蛋白与Cajal体的相互作用不同，并揭示了有丝分裂后核仁和Cajal体重组途径的相似之处。

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