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首页> 外文期刊>Journal of combinatorial chemistry >Combinatorial materials research applied to the development of new surface coatings I: A multiwell plate screening method for the high-throughput assessment of bacterial biofilm retention on surfaces
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Combinatorial materials research applied to the development of new surface coatings I: A multiwell plate screening method for the high-throughput assessment of bacterial biofilm retention on surfaces

机译：组合材料研究应用于新表面涂层的开发I：一种多孔板筛选方法，用于高通量评估表面细菌生物膜的保留

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摘要

Combinatorial, high-throughput capabilities have been established to aid in the rapid development of new and effective antifouling marine coatings for naval applications. A biological screening process involving marine bacteria was developed that allows for rapid and effective quantification of bacterial biofilm growth and retention on large numbers of coating surfaces in parallel. The screening process involves (1) multiwell plate modifications for coating deposition, (2) deposition of combinatorial coating libraries via an automated liquid dispensing robot, (3) coating thickness measurements of cured coatings, (4) preconditioning of coatings via immersion in deionized water, (5) bacterial incubation, (6) plate processing, and (7) data analysis for identification of promising candidates. The details of the method developed are described in this document.
机译：已经建立了组合的高通量功能，以帮助快速开发适用于海军的新型有效防污船用涂​​料。开发了一种涉及海洋细菌的生物筛选方法，该方法可以快速有效地定量细菌生物膜的生长以及平行在大量涂层表面上的保留。筛选过程涉及（1）用于涂层沉积的多孔板修饰，（2）通过自动液体分配机器人沉积组合涂层库，（3）固化涂层的涂层厚度测量，（4）通过浸入去离子水中进行预处理，（5）细菌培养，（6）平板处理和（7）数据分析，以确定有前途的候选基因。本文档中介绍了所开发方法的详细信息。

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