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首页> 外文期刊>Journal of cellular and molecular medicine. >Neural stem cells improve neuronal survival in cultured postmortem brain tissue from aged and Alzheimer patients.
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Neural stem cells improve neuronal survival in cultured postmortem brain tissue from aged and Alzheimer patients.

机译：神经干细胞可改善老年和阿尔茨海默氏病患者培养的死后脑组织中神经元的存活率。

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摘要

Neurodegenerative diseases are progressive and incurable and are becoming ever more prevalent. To study whether neural stem cell can reactivate or rescue functions of impaired neurons in the human aging and neurodegenerating brain, we co-cultured postmortem slices from Alzheimer patients and control participants with rat embryonic day 14 (E14) neural stem cells. Viability staining based on the exclusion of ethidium bromide by intact plasma membranes showed that there were strikingly more viable cells and fewer dead cells in slices co-cultured with neural stem cells than in untreated slices. The presence of Alzheimer pathology in the brain slices did not influence this effect, although the slices from Alzheimer patients, in general, contained fewer viable cells. Co-culturing with rat E14 fibroblasts did not improve the viability of neurons in the human brain slices. Since the human slices and neural stem cells were separated by a membrane during co-culturing our data show for the first time that neural stem cells release diffusible factors that may improve the survival of aged and degenerating neurons in human brains.
机译：神经退行性疾病是进行性和不可治愈的，并且变得越来越普遍。为了研究神经干细胞是否可以在人类衰老和神经退化的大脑中重新激活或恢复受损神经元的功能，我们将来自阿尔茨海默氏病患者和对照组参与者的死后切片与大鼠胚胎第14天（E14）神经干细胞共培养。基于完整质膜排除溴化乙锭的活力染色显示，与神经干细胞共培养的切片中，与未处理的切片相比，存在显着更多的存活细胞和更少的死细胞。尽管通常来说，来自阿尔茨海默氏病患者的切片包含的活细胞较少，但脑切片中存在的阿尔茨海默氏病病理并未影响这种效果。与大鼠E14成纤维细胞共培养不能改善人脑切片中神经元的活力。由于在共同培养过程中人的切片和神经干细胞被膜分开，我们的数据首次表明神经干细胞释放出可扩散的因子，这些因子可改善人脑中衰老和退化的神经元的存活率。

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